Integrin beta 8 Antibody (416922) [Unconjugated]

WM-115 human melanoma cell line was stained with Human Integrin beta 8 Monoclonal Antibody (Catalog # MAB4775, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 416922
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 8 Antibody (416922) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Integrin beta 8
  Glu43-Arg684 (predicted)
  Accession # P26012
• Specificity: Detects human Integrin beta 8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Integrins beta 1, beta 2, beta 3, beta 4, beta 5, beta 6, beta 7, or recombinant mouse Integrin beta 8 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 8 Antibody (416922) [Unconjugated]

• Integrin beta 8
• integrin beta-8
• integrin, beta 8
• ITGB8

Background

Integrin beta 8 (Integrin beta 8) is a 90 kDa type I transmembrane glycoprotein of the Integrin family of adhesion molecules. It associates with Integrin alpha V to form a receptor for vitronectin, fibrin, and the latency associated peptide (LAP). Binding to LAP promotes the proteolytic release of active TGF-beta from LAP. Integrin alpha V beta 8 is required for vascular morphogenesis in the embryonic brain and yolk sac. Within the extracellular domain, human Integrin  beta 8 shares 87% aa sequence identity with mouse and rat Integrin  beta 8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 8 Antibody (MAB4775)(4)

Publications using MAB4775

• Rouaud, L;Baudin, L;Gautier-Isola, M;Van Meerbeeck, P;Feyereisen, E;Blacher, S;van Baren, N;Kridelka, F;Lucas, S;Noel, A; Spatial Distribution of Non-Immune Cells Expressing Glycoprotein A Repetitions Predominant in Human and Murine Metastatic Lymph Nodes Cancers 2023-11-28 [PMID: 38067325] (Flow Cytometry, Human)
• Changlin Yang, Guimei Tian, Mariana Dajac, Andria Doty, Shu Wang, Ji-Hyun Lee et al. Slow-Cycling Cells in Glioblastoma: A Specific Population in the Cellular Mosaic of Cancer Stem Cells Cancers (Basel) 2/23/2022 [PMID: 35267434]
• I Malenica, J Adam, S Corgnac, L Mezquita, E Auclin, I Damei, L Grynszpan, G Gros, V de Montpré, D Planchard, N Théret, B Besse, F Mami-Choua Integrin-alphaV-mediated activation of TGF-beta regulates anti-tumour CD8 T cell immunity and response to PD-1 blockade Nature Communications, 2021-09-01;12(1):5209. 2021-09-01 [PMID: 34471106] (Flow Cytometry, Human)
• Highly invasive melanoma cells activate the vascular endothelium via an MMP-2/integrin alphavbeta5-induced secretion of VEGF-A. Am. J. Pathol., 2012-05-31;181(2):693-705. 2012-05-31 [PMID: 22659470] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGB8
• Uniprot:
  • P26012()