Integrin beta 8 Antibody (2B4) - Azide and BSA Free

Western Blot: Integrin beta 8 Antibody (2B4) [H00003696-M01] - Analysis of ITGB8 expression in transfected 293T cell line by ITGB8 monoclonal antibody (M01), clone 2B4.Lane 1: ITGB8 transfected lysate(85.6 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: Integrin beta 8 Antibody (2B4) [H00003696-M01] - Analysis of ITGB8 transfected lysate using anti-ITGB8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ITGB8 MaxPab rabbit polyclonal antibody.

ELISA: Integrin beta 8 Antibody (2B4) [H00003696-M01] - Detection limit for recombinant GST tagged ITGB8 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, Func, IP
• Clone: 2B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Integrin beta 8 Antibody (2B4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ITGB8 (NP_002205, 392 a.a. ~ 503 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VENQVQGIYFNITAICPDGSRKPGMEGCRNVTSNDEVLFNVTVTMKKCDVTGGKNYAIIKPIGFNETAKIHIHRNCSCQCEDNRGPKGKCVDETFLDSKCFQCDENKCHFDE
• Specificity: ITGB8 (2B4)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Functional
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Integrin beta 8 Antibody (2B4) - Azide and BSA Free

• Integrin beta 8
• integrin beta-8
• integrin, beta 8
• ITGB8

Background

This gene is a member of the integrin beta chain family and encodes a single-pass type I membrane protein with a VWFA domain and four cysteine-rich repeats. This protein noncovalently binds to an alpha subunit to form a heterodimeric integrin complex. In general, integrin complexes mediate cell-cell and cell-extracellular matrix interactions and this complex plays a role in human airway epithelial proliferation. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 8 Antibody (H00003696-M01)(1)

Publication using H00003696-M01

• Ballana E, Pauls E, Clotet B et al. beta5 integrin is the major contributor to the alphaVintegrin-mediated blockade of HIV-1 replication. J Immunol. 2010-11-22 [PMID: 21098231]

Bioinformatics

• Gene Symbol: ITGB8
• Entrez:
  • 3696(Human)
• OMIM:
  • 604160(Human)
• Uniprot:
  • NP_002205()