Integrin beta 6 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Integrin beta 6 Antibody [NBP2-14136] - Staining of human cell line RT4 shows localization to nucleoplasm & cell junctions. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Integrin beta 6 Antibody [NBP2-14136] - Staining of human rectum shows strong cytoplasmic and membranous positivity in granular pattern in glandular cells.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Integrin beta 6 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: RGDCVCGKCVCTNPGASGPTCERCPTCGDPCNSKRSCIECHLSAAGQAREECVDKCKLAGATISEEEDFSKDGSVSCSLQGENECLITFLITTDNEGKTIIHSI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITGB6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
• Control Peptide:

  Integrin beta 6 Protein (NBP2-14136PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24305999). Rat (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Integrin beta 6 Antibody - BSA Free

• Integrin beta 6
• integrin beta-6
• integrin, beta 6
• ITGB6

Background

Integrin alpha-V/beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 6 Antibody (NBP2-14136)(2)

Publications using NBP2-14136

• Goodman SL. The path to VICTORy - a beginner's guide to success using commercial research antibodies J. Cell. Sci. 2018-05-15 [PMID: 29764917] (IF/IHC)
• Wang SK, Choi M, Richardson AS et al. ITGB6 loss-of-function mutations cause autosomal recessive amelogenesis imperfecta. Hum Mol Genet 2014-04-15 [PMID: 24305999] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: ITGB6