Integrin beta 6 Antibody (CSbeta6) - Azide and BSA Free

• Reactivity: Hu, Rt, Pm, Rb
• Applications: Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: CSbeta6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Integrin beta 6 Antibody (CSbeta6) - Azide and BSA Free Summary

• Description: Relevant Entrez Gene Numbers: NM_000888.3
• Immunogen: beta 6 knockout mice have been immunized with recombinant secreted human alpaVbeta6.
• Specificity: Monoclonal antibody MAB2076Z, raised by immunizing beta6 knockout mice with recombinant secreted human alphavbeta6, is useful for flow cytometry and immunoprecipitation in all species tested, including mouse. It also works well for immunohistochemistry of acetone-fixed tissues in several species, including rat, rabbit, monkey, and human. The antibody does not work well for immunohistochemistry of mouse tissue. This antibody does not recognize the aV subunit and does not react with alphaV integrins other than alphavbeta6. Because the antibody is not effective in western Blot, it is unknown whether the epitope recognized by the antibody localizes to the alphavbeta6 heterodimer or to the beta6 subunit only.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: Immunohistochemistry (acetone fixation): Effective for Rabbit, rat, monkey, and human. Not effective for mouse tissue. Immunocytochemistry/Immunofluorescence : Effective for F6, HT-29, H441 cell lines (others not tested). Flow cytometry: Effective for all species tested, including rabbit, rat, monkey, human, and mouse. Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_000888.3

Alternate Names for Integrin beta 6 Antibody (CSbeta6) - Azide and BSA Free

• Integrin beta 6
• integrin beta-6
• integrin, beta 6
• ITGB6

Background

Integrin alpha-V/beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 6 Antibody (NBP2-29800)(1)

Publication using NBP2-29800

• Kawamoto E, Nago N, Okamoto T et al. The Lectin-Like Domain of Thrombomodulin Inhibits beta 1 Integrin-Dependent Binding of Human Breast Cancer-Derived Cell Lines to Fibronectin Biomedicines 2021-02-07 [PMID: 33562346] (FLOW, Human)

Bioinformatics

• Gene Symbol: ITGB6
• Uniprot:
  • P18564()