Integrin alpha V/CD51 Antibody (P2W7) [Alexa Fluor® 488]

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin aV/CD51 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1219G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: P2W7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Integrin alpha V/CD51 Antibody (P2W7) [Alexa Fluor® 488] Summary

• Immunogen: Ocular melanoma cell line
• Specificity: Detects the ectodomain of human Integrin alpha V/CD51.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAV
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha V/CD51 Antibody (P2W7) [Alexa Fluor® 488]

• antigen identified by monoclonal L230
• CD51 antigen
• CD51
• Integrin alpha V
• integrin alpha-V
• integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• ITGAV
• MSK8
• Vitronectin Receptor alpha
• Vitronectin receptor subunit alpha
• VNRA
• VNRADKFZp686A08142

Background

Integrin alpha V (CD51), also known as Vitronectin Receptor subunit alpha, is a 140-150 kDa member of the integrin alpha -chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta -chains, including beta 1, 3, 5, 6 and 8. Human  alpha V is a 1018 amino acid (aa) type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) (aa 31-992), and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta -propeller domain (aa 46-483). Furin cleavage of the alpha V ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2 and TSP. The ECD of human alpha V is 92% aa identical to mouse alpha V ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha V/CD51 Antibody (FAB1219G)(2)

Publications using FAB1219G

• TS Stantchev, A Zack-Taylo, N Mattson, K Strebel, CC Broder, KA Clouse Cytokine Effects on the Entry of Filovirus Envelope Pseudotyped Virus-Like Particles into Primary Human Macrophages Viruses, 2019-09-23;11(10):. 2019-09-23 [PMID: 31547585] (Flow Cytometry, Human)
• FJ Zapatero-B, E Dietzel, O Dolnik, K Döhner, R Costa, B Hertel, B Veselkova, J Kirui, A Klintworth, MP Manns, S Pöhlmann, T Pietschman, T Krey, S Ciesek, G Gerold, B Sodeik, S Becker, T von Hahn Characterization of the Filovirus-Resistant Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors Viruses, 2019-03-19;11(3):. 2019-03-19 [PMID: 30893855] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGAV