Integrin alpha L/CD11a Antibody (CR38)

Human peripheral blood monocytes were stained with Human Integrin alpha L/CD11a Monoclonal Antibody (Catalog # MAB35951, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: CR38
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha L/CD11a Antibody (CR38) Summary

• Immunogen: Fibronectin purified human monocytes
• Specificity: Detects human Integrin alpha L/CD11a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Integrin alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6, alpha 8, alpha 9, alpha 10, alpha 11, alpha M, alpha V, alpha X, rhIntegrin beta 3, rhIntegrin alpha 6(x1)+ beta 4, alpha 7(x2)+ beta 1, alpha D+ beta 2, alpha E+ beta 7, recombinant mouse Integrin alpha 2b or alpha L is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAL
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha L/CD11a Antibody (CR38)

• CD11 antigen-like family member A
• CD11a antigen
• CD11a
• CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide
• Integrin alpha L
• integrin alpha-L
• integrin gene promoter
• integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide)
• ITGAL
• Leukocyte adhesion glycoprotein LFA-1 alpha chain
• Leukocyte function-associated molecule 1 alpha chain
• LFA-1 alpha
• LFA-1
• LFA1A
• LFA-1A
• Ly-15
• Ly-21
• lymphocyte function-associated antigen 1

Background

Integrin subunit alpha L/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin  beta 2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin alpha L contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alpha L ECD share 74% aa identity. A second isoform has a 53 aa insert in the ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha L/CD11a Antibody (MAB35951)(1)

Publication using MAB35951

• LC Ristow, V Tran, KJ Schwartz, L Pankratz, A Mehle, JD Sauer, RA Welch The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity MBio, 2019;10(4):. 2019 [PMID: 31289186] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ITGAL