Integrin alpha E/CD103 Antibody (Ber-ACT8) [FITC]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [FITC] [NBP1-97568] - Surface staining of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) FITC.

• Reactivity: Hu, Pm
• Applications: WB, Flow, IHC, IP
• Clone: Ber-ACT8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

Integrin alpha E/CD103 Antibody (Ber-ACT8) [FITC] Summary

• Additional Information: Clone Ber-ACT8 was used by HLDA to establish CD designation.
• Immunogen: HTLV-1 induced human T cell line MAPS16
• Specificity: The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes. HLDA V; WS Code A067
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAE
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 4ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS and 0.2% (w/v) high grade BSA (protease free)
• Preservative: 0.09% Sodium Azide
• Purity: Size Exclusion Chromatography

Alternate Names for Integrin alpha E/CD103 Antibody (Ber-ACT8) [FITC]

• antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide
• CD103 antigen
• CD103
• HML-1 antigen
• HUMINAE
• Integrin alpha E
• integrin alpha-E
• Integrin alpha-IEL
• integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide)
• ITGAE
• MGC141996
• Mucosal lymphocyte 1 antigen

Background

CD103 / Integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activat

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAE