Integrin alpha E/CD103 Antibody (AP-MAB0828)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP, CyTOF-ready
Clone
AP-MAB0828
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Integrin alpha E/CD103 Antibody (AP-MAB0828) Summary

Immunogen
Mouse mammary tumor cells
Specificity
Integrin alpha IEL chain
Isotype
IgG2
Clonality
Monoclonal
Host
Rat
Gene
ITGAE
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
Flow Cytometry, Immunoprecipitation, Immunohistochemistry-Frozen and Inhibition of CD103 mediated adhesion

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS - Azide free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Integrin alpha E/CD103 Antibody (AP-MAB0828)

  • antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide
  • CD103 antigen
  • CD103
  • HML-1 antigen
  • HUMINAE
  • Integrin alpha E
  • integrin alpha-E
  • Integrin alpha-IEL
  • integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide)
  • ITGAE
  • MGC141996
  • Mucosal lymphocyte 1 antigen

Background

CD103 has a unique and fairly restricted tissue distribution. It is expressed on almost all intestinal intraepithelial lymphocytes (IEL), on dendritic epidermal T cells (DEC), on subpopulations of peripheral T cells, and on distinct subsets of fetal, neonatal, and adult thymocytes. E-cadherin is the epithelial-cell ligand for CD103. The ordered expression of alpha IEL during thymocyte development (which occurs under the influence of the thymic epithelium), the high level of expression of alphaIEL on those peripheral T cells found in epithelial tissues (IEL and DEC), and the expression of CD103 on a subset of CD8+ lymphocytes responding to allogeneic epithelial cells suggest that CD103 may have a common role in the interactions of T lymphocytes with epithelia during T-cell maturation and effector functions. This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with mouse mammary tumor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Integrin alpha E/CD103 Antibody (NBP1-06674). (Showing 1 - 1 of 1 FAQ).

  1. We would be interested in the following antibody from your company: NBP1-06674 However, it is not exactly clear to us, what your company means when it says mouse mammary tumor cells were used as immunogen. Does this mean that tumor cells overexpressing CD103 were used? Since mouse mammary tumor cells per se are not really an appropriate immunogen.
    • Our Integrin alpha E antibody NBP1-06674 was created using mouse mammary tumor cells known to overexpress the ITGAE protein. These were selected and cultured to produce this monoclonal. We fully guarantee this antibody to detect mouse ITGAE protein in FLOW, IHC-frozen tissues, and immunoprecipitation.

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Bioinformatics

Gene Symbol ITGAE
Entrez