Integrin alpha 6/CD49f Antibody (CLB-701)

Product Discontinued

Integrin alpha 6/CD49f Antibody (CLB-701) Summary

• Immunogen: The antibody reacts with the CD49f antigen.
• Specificity: The monoclonal antibody is directed against the CD49f antigen, molecular weight 30/31, 120 kD (GP Ic or VLA-6 alpha-chain), which can form distinct complexes with either the CD29 antigen (GP IIa or VLA beta chain), resulting in the VLA-6 (alpha-6, beta-1) complex which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6, beta-4 complex which is expressed on various human epithelial cells. Platelet VLA-6 functions as a laminin receptor and platelet adhesion to laminin is inhibited by this antibody. The monoclonal antibody reacts with platelets, megakaryocytes, T-lymphocytes and common acute lymphoblastic leukemia cells (alpha-6, beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA6
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
• Application Notes: Functional studies on VLA-6.The antibody is useful for indirect immunofluorescence staining with analysis by cytofluoro-metry or fluorescence microscopy and immunohistology.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 20mM Tris (pH 8.0), 0.15M NaCl, 10 mg BSA and 0.001% Merthiolate
• Preservative: No Preservative
• Purity: Ion exchange chromatography

Alternate Names for Integrin alpha 6/CD49f Antibody (CLB-701)

• CD49 antigen-like family member F
• CD49f antigen
• CD49f
• DKFZp686J01244
• FLJ18737
• Integrin alpha 6
• integrin alpha-6
• integrin alpha6B
• integrin, alpha 6
• ITGA6
• ITGA6B
• Platelet gpl
• VLA-6

Background

Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Integrin alpha 6 complexes are receptors for laminins, which are components of basement membranes. Integrin alpha 6 complexes may play an important role in embryogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA6