Integrin alpha 5/CD49e Antibody (HMa5-1) [FITC]

Flow Cytometry: Integrin alpha 5/CD49e Antibody (HMa5-1) [FITC] [NBP1-44001] - Staining of BALB/c bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.25 micrograms of Armenian Hamster IgG Isotype Control FITC (left) or 0.5 micrograms of Anti-Mouse/Rat CD49e (Integrin a5) FITC (right). Total viable cells were used for analysis.

Product Discontinued

Integrin alpha 5/CD49e Antibody (HMa5-1) [FITC] Summary

• Immunogen: The immunogen for this antibody was CD49e.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: ITGA5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This HMa5-1 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24256316)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin alpha 5/CD49e Antibody (HMa5-1) [FITC]

• CD49 antigen-like family member E
• CD49e antigen
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha subunit
• FNRAVLA5A
• Integrin alpha 5
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5
• very late activation protein 5, alpha subunit
• VLA-5 alpha
• VLA-5

Background

The HMalpha5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin alpha5 or VLA-5A. CD49e is a member of the integrin alpha chain family, and binds with CD29 (integrin beta1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The HMalpha5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates. Furthermore, HMalpha5-1 injected into mice subcutaneously, along with anti-CD49d and anti-CD61, is able to inhibit passive cutaneous anaphylaxis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA5