Flow Cytometry: Integrin alpha 5/CD49e Antibody (HMa5-1) [NBP1-43401] - Analysis using the FITC conjugate of NBP1-43401. Staining of BALB/c bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.25 micrograms of ...read more
Flow Cytometry: Integrin alpha 5/CD49e Antibody (HMa5-1) [NBP1-43401] - Staining of C57Bl/6 bone marrow cells with 0.5 ug of Armenian Hamster IgG Isotype Control Purified (left) or 0.5 ug of Anti-Mouse/Rat CD49e ...read more
This HMa5-1 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24256316)
Read Publication using NBP1-43401 in the following applications:
The HMalpha5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin alpha5 or VLA-5A. CD49e is a member of the integrin alpha chain family, and binds with CD29 (integrin beta1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The HMalpha5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Integrin alpha 5/CD49e Antibody (HMa5-1) and receive a gift card or discount.