Flow Cytometry: Integrin alpha 5/CD49e Antibody (HMa5-1) [NBP1-43401] - Analysis using the FITC conjugate of NBP1-43401. Staining of BALB/c bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.25 micrograms of ...read more
Flow Cytometry: Integrin alpha 5/CD49e Antibody (HMa5-1) [NBP1-43401] - Staining of C57Bl/6 bone marrow cells with 0.5 ug of Armenian Hamster IgG Isotype Control Purified (left) or 0.5 ug of Anti-Mouse/Rat CD49e ...read more
Aqueous buffer, may contain carrier protein/stabilizer (proprietary).
0.09% Sodium Azide
Protein A or G purified
This HMa5-1 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24256316)
Read Publication using NBP1-43401 in the following applications:
The HMalpha5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin alpha5 or VLA-5A. CD49e is a member of the integrin alpha chain family, and binds with CD29 (integrin beta1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The HMalpha5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.