Integrin alpha 5/CD49e Antibody (HMa5-1) - BSA Free

Flow Cytometry: Integrin alpha 5/CD49e Antibody (HMa5-1) [NBP1-43401] - Analysis using the FITC conjugate of NBP1-43401. Staining of BALB/c bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.25 micrograms of Armenian Hamster IgG Isotype Control FITC (left) or 0.5 micrograms of Anti-Mouse/Rat CD49e (Integrin a5) FITC (right). Total viable cells were used for anal

Flow Cytometry: Integrin alpha 5/CD49e Antibody (HMa5-1) [NBP1-43401] - Staining of C57Bl/6 bone marrow cells with 0.5 ug of Armenian Hamster IgG Isotype Control Purified (left) or 0.5 ug of Anti-Mouse/Rat CD49e (Integrin alpha 5) Purified (right) followed by Anti-Armenian Hamster IgG FITC

Product Discontinued

Integrin alpha 5/CD49e Antibody (HMa5-1) - BSA Free Summary

• Immunogen: The immunogen for this antibody was CD49e.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: ITGA5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: This HMa5-1 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24256316)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin alpha 5/CD49e Antibody (HMa5-1) - BSA Free

• CD49 antigen-like family member E
• CD49e antigen
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha subunit
• FNRAVLA5A
• Integrin alpha 5
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5
• very late activation protein 5, alpha subunit
• VLA-5 alpha
• VLA-5

Background

The HMalpha5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin alpha5 or VLA-5A. CD49e is a member of the integrin alpha chain family, and binds with CD29 (integrin beta1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The HMalpha5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 5/CD49e Antibody (NBP1-43401)(1)

Publication using NBP1-43401

• Wakatsuki S, Araki T, Sehara-Fujisawa A. Neuregulin-1/glial growth factor stimulates Schwann cell migration by inducing alpha5 beta1 integrin-ErbB2-focal adhesion kinase complex formation. Genes Cells. 2013-11-20 [PMID: 24256316] (ICC/IF, Mouse, Rat)

Bioinformatics

• Gene Symbol: ITGA5