Insulin R/CD220 Antibody (4B2) - BSA Free

Immunocytochemistry/Immunofluorescence: Insulin R/CD220 Antibody (4B2) [NBP3-26192] - Staining of MCF7 Cells with Insulin R/CD220 Antibody (4B2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

• Reactivity: Hu
• Applications: ELISA, ICC/IF
• Clone: 4B2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Insulin R/CD220 Antibody (4B2) - BSA Free Summary

• Description: Novus Biologicals Rabbit Insulin R/CD220 Antibody (4B2) - BSA Free (NBP3-26192) is a recombinant monoclonal antibody validated for use in ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human Insulin R/CD220 [UniProt P06213]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: INSR
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Insulin R/CD220 Antibody (4B2) - BSA Free

• CD 220
• CD220 antigen
• CD220
• EC 2.7.10
• EC 2.7.10.1
• HHF5
• INSR
• Insulin R
• insulin receptor
• InsulinR
• IR

Background

Insulin receptor plays a major part in the regulation of glucose homeostasis, which when malfunctioning can result in diabetes and cancer. Encoded by the INSR gene, insulin receptor has two isoforms produced by alternative splicing, HIR-B and HIR-A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INSR