Human INSL3 ELISA Kit (Colorimetric)

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ELISA: Human INSL3 ELISA Kit (Colorimetric) [NBP2-81140] - Samples were spiked with high concentrations of Human INSL3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human INSL3 ELISA Kit (Colorimetric) [NBP2-81140] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

Order Details

Human INSL3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human INSL3 in samples. No significant cross-reactivity or interference between Human INSL3 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.48%
Intra-Assay
CV% < 5.18%
Spike Recovery
92-109%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
INSL3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human INSL3 ELISA Kit (Colorimetric)

  • INSL3
  • insulin-like 3 (Leydig cell)
  • insulin-like 3
  • Ley I-L
  • leydig insulin -like hormone
  • leydig insulin -like peptide
  • Leydig insulin-like peptide
  • Ley-I-L
  • MGC119818
  • MGC119819
  • relaxin-like factor b
  • Relaxin-like factor
  • RLF
  • RLFley-I-L
  • RLNL

Background

Insulin-like 3 (INSL3), a member of the insulin-like hormone superfamily, is specifically expressed in Leydig cells of the fetal and postnatal testis and in theca cells of the postnatal ovary. It is synthesized as a 131-amino acid preproprotein, which contains a 24-amino acid signal peptide. The human INSL3 gene is assigned to bands p13.2-p12 of the short arm of chromosome 19 with the similar organization to that of insulin and relaxin. INSL3 induces gubernaculum development in an androgen-independent way, while androgen-mediated regression of the CSL occurs independently from Insl3. Moreover, INSL3 is a ligand for LGR8 and INSL3-LGR8 mutations are believed to be associated with human cryptorchidism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for INSL3 ELISA Kit (NBP2-81140). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol INSL3