Relaxin R2/LGR8 Antibody

Images

 
Immunohistochemistry-Paraffin: Relaxin R2/LGR8 Antibody [NLS4753] - Analysis of anti-RXFP2 / LGR8 antibody with human breast.
Immunohistochemistry: Relaxin R2/LGR8 Antibody [NLS4753] - Analysis of anti-RXFP2 / LGR8 antibody with human uterus.
Immunohistochemistry-Paraffin: Relaxin R2/LGR8 Antibody [NLS4753] - Analysis of anti-RXFP2 / LGR8 antibody with human brain, glioblastoma.

Product Details

Summary
Reactivity Hu, Rt, Eq, Mk, PmSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Relaxin R2/LGR8 Antibody Summary

Immunogen
Synthetic 14 amino acid peptide from 2nd cytoplasmic domain of human RXFP2.
Localization
Cell Membrane
Specificity
Human RXFP2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Monkey (93%), Primate (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
RXFP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:10000
  • Immunohistochemistry-Paraffin 26 - 130 ug/ml
Application Notes
WB: Mouse tissue has been controversial. ICC/IF usage is reported in scientific literature (PMID: 24169563).
Positive Control
Relaxin R2/LGR8 Lysate (NBL1-15638)
Reviewed Applications
Read 1 Review rated 4
using
NLS4753 in the following applications:

Publications
Read Publication using
NLS4753 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoste (100%), Panda (93%), Canine (86%), Porcine (86%), Xenopus (86%). Rat reactivity reported in scientific literature (PMID: 24169563).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Relaxin R2/LGR8 Antibody

  • G protein coupled receptor affecting testicular descent
  • Gpr106
  • GPR106G-protein coupled receptor 106
  • Great
  • GREATRelaxin family peptide receptor 2
  • INSL3R
  • leucine-rich repeat-containing G protein-coupled receptor 8
  • Leucine-rich repeat-containing G-protein coupled receptor 8
  • Lgr8
  • LGR8.1
  • LGR8G-protein coupled receptor affecting testicular descent
  • Relaxin R2
  • relaxin receptor 2
  • relaxin/insulin-like family peptide receptor 2
  • RelaxinR2
  • RLN2R
  • Rxfp2
  • RXFPR2

Background

LGR8 is a member of the Relaxin Receptor subfamily. LGR8 has been shown to be important for testis descent. LGR8 has been reported in blood, bone marrow, brain, kidney, muscle, testis, thyroid, and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Relaxin R2/LGR8 Antibody (NLS4753)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: ICC/IF, IHC-P.


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ICC/IF
(1)
IHC-P
(1)
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Rat
(1)
All Species

Review for Relaxin R2/LGR8 Antibody (NLS4753) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NLS4753:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Carol Hanna
WB Other 07/27/2010
View

Summary

ApplicationWestern Blot
Sample TestedProtein lysate for lung, ovary, pituitary, testis, hypothalamus, Sample Amount: 80ug
SpeciesOther
CommentsThe antibody worked for WB, however there was no information available on dilutions or expected size of band. We found 1:500 worked best for monkey tissue.

Blocking

Blocking DetailsBlocking Buffer: Invitrogen Western Dot 625 kit blocking buffer, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:500, Incubation Dilution Buffer: Wash buffer w/ WesternDot kit, Incubation Time: overnight, Incubation Temp:4C

Secondary Antibody

Secondary DescriptionSecondary Ab: biotin conjugated (see above kit), Secondary Ab Dilution Ratio: 1:2000
Secondary Manufacturer Cat#W10142

Details

Detection NotesWesternDot, Exposure Time: 3sec, Pos Ctrl: lysate (did not work), Neg Ctrl:none, Specific Bands:86kDa, Non-Specific Bands:120kDa

Comments

CommentsThe antibody worked for WB, however there was no information available on dilutions or expected size of band. We found 1:500 worked best for monkey tissue.

Product General Protocols

View specific protocols for Relaxin R2/LGR8 Antibody (NLS4753): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Relaxin R2/LGR8 Antibody (NLS4753) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Carol Hanna
07/27/2010
Application: WB
Species: Other

Bioinformatics

Gene Symbol RXFP2
Entrez