Inorganic Pyrophosphatase/PPA1 Antibody (3B2) - Azide and BSA Free

Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01] - PPA1 monoclonal antibody (M01), clone 3B2 Analysis of PPA1 expression in A-431.

Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01] - PPA1 monoclonal antibody (M01), clone 3B2. Analysis of PPA1 expression in PC-12.

Sandwich ELISA: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01] - Detection limit for recombinant GST tagged PPA1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clone: 3B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Inorganic Pyrophosphatase/PPA1 Antibody (3B2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PPA1 (NP_066952, 10 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVFHMVVEVPRWSNAKMEIATKDPLNPIKQDVKKGKLRYVANLFPYKGYIWNYGAIPQTWEDPGHNDKHT
• Localization: Cytoplasmic
• Specificity: PPA1 - pyrophosphatase (inorganic) 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPA1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Inorganic Pyrophosphatase/PPA1 Antibody (3B2) - Azide and BSA Free

• diphosphate phosphohydrolase
• EC 3.6.1.1
• inorganic diphosphatase
• inorganic pyrophosphatase 1
• inorganic pyrophosphatase
• IOPPP
• IOPPPMGC111556
• IPP1
• PP1
• PPA1
• PPase
• PPcytosolic inorganic pyrophosphatase
• pyrophosphatase (inorganic) 1
• pyrophosphatase (inorganic)
• pyrophosphatase 1
• Pyrophosphate phospho-hydrolase
• SID6-8061

Background

The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPA1
• Entrez:
  • 5464(Human)
• OMIM:
  • 179030(Human)
• Uniprot:
  • NP_066952(Human)