INMT Antibody (31G7) - BSA Free

Immunohistochemistry: INMT Antibody (31G7) [NBP3-26197] - Image of INMT Antibody (31G7) diluted at 1:50 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.24% DAB.

Flow Cytometry: INMT Antibody (31G7) [NBP3-26197] - Overlay Peak curve showing SH-SY5Y cells stained with INMT Antibody (31G7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: ELISA, Flow, IHC
• Clone: 31G7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

INMT Antibody (31G7) - BSA Free Summary

• Description: Novus Biologicals Rabbit INMT Antibody (31G7) - BSA Free (NBP3-26197) is a recombinant monoclonal antibody validated for use in IHC, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human INMT [UniProt O95050]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: INMT
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Immunohistochemistry 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for INMT Antibody (31G7) - BSA Free

• Amine N-methyltransferase
• Aromatic alkylamine N-methyltransferase
• Arylamine N-methyltransferase
• EC 2.1.1
• EC 2.1.1.49
• Indolamine N-methyltransferase
• indolethylamine N-methyltransferase
• MGC125940
• MGC125941
• nicotine N-methyltransferase

Background

INMT is a gene which codes for a protein that functions as a thioether S-methyltransferase which is necessary in the detoxification of selenium compounds and the catalyzation of the N-methylations of tryptamine and compounds with similar structures and has two isoforms with lengths of 263 and 262 amino acids, that both have a weight of approximately 29 kDa. Current studies are being done on diseases and disorders relating to this gene including appendicitis, homosydteine, thryoditis, and carcinoma. INMT has also been shown to interact with KCNMA1, FMO1, IDO1, AANAT, and ABP1 in pathways such as the tryptophan metabolism and selenocompound metabolism pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INMT