Inhibin beta A Antibody

Images

 
Western Blot: Inhibin beta A Antibody [NB110-61019] - Analysis in A549 cells were subjected to SDS PAGE followed by western blot at dilution of 1:1000
Immunocytochemistry/ Immunofluorescence: Inhibin beta A Antibody [NB110-61019] - Analysis of A549 cells at dilution of 1:50
Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NB110-61019] - IHC analysis of Inhibin beta A in mouse colon tissue sections. Image courtesy of product review submitted by Linda Gutierrez.
Western Blot: Inhibin beta A Antibody [NB110-61019] - Mouse brain were subjected to SDS PAGE followed by western blot with affinity purified Ab at dilution of 1:800.
Immunocytochemistry/ Immunofluorescence: Inhibin beta A Antibody [NB110-61019] - Analysis of Hela cells.
Immunocytochemistry/ Immunofluorescence: Inhibin beta A Antibody [NB110-61019] - Analysis of A549 cells at dilution of 1:50
Immunohistochemistry: Inhibin beta A Antibody [NB110-61019] - Immunohistochemical staining of formalin-fixed paraffin-embedeed prostate cancer showing Extracellular staining, with affinity purified Ab. Dilution: 1:100.
Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NB110-61019] - Immunohistochemistry of paraffin-embedded human lung cancer at Dilution 1:50 (under 40x lens).
Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NB110-61019] - Immunohistochemistry of paraffin-embedded human lung cancer at Dilution 1:50 (under 10x lens).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, B/N, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Inhibin beta A Antibody Summary

Immunogen
INHBA fusion protein ag1001
Specificity
Inhibin Beta-1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
INHBA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:000
  • Blocking/Neutralizing
  • ELISA 1:1000-1:100000
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
Tested in Human, Mouse, and Rat. Other species are not tested.Blocking/Neutralizing was reported in scientific literature. Please decide the specificity by homology.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB110-61019 in the following applications:

Publications
Read Publications using
NB110-61019 in the following applications:

  • B/N
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3) and 50% Gycerol
Preservative
0.1% Sodium Azide
Concentration
0.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Inhibin beta A Antibody

  • Activin beta-A chain
  • beta A (activin A, activin AB alpha polypeptide)
  • EDF
  • erythroid differentiation factor
  • Erythroid differentiation protein
  • follicle-stimulating hormone-releasing protein
  • FRP
  • FSH-releasing protein
  • inhibin beta A chain
  • inhibin, beta A
  • Inhibin, beta-1

Background

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Inhibin beta A Antibody (NB110-61019)(3)

Review for Inhibin beta A Antibody (NB110-61019) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-P.

Reviews using NB110-61019:
Filter by Applications
IHC-P
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Inhibin beta A NB110-61019
Enlarge
reviewed by:
Anonymous
IHC-P Mouse 07/23/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse tissue section from colon
SpeciesMouse
Lot7112012
CommentsIt might work better with a dilution of 1/200.

Blocking

Blocking DetailsHorse serum for 30 minutes

Primary Anitbody

Dilution Ratio1/100 dilution, overnight incubation in the fridge. Diluted in PBS

Secondary Antibody

Secondary DescriptionImmPRESS TM Anti-Rabbit Ig (peroxidase)
Secondary Manufacturer Cat#MP-7401
Secondary ConcentrationBecause it is already diluted, we applied it directly on the slides for 30 minutes before rinsing and using the chromogen DAB

Details

Detection NotesImpress anti-rabbit reagent (Vector Labs) for 30 minutes.
Fixation DetailsHistochoice fixation (no antigen retrieval)
Wash DescriptionPBS three times

Comments

CommentsIt might work better with a dilution of 1/200.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Inhibin beta A Antibody (NB110-61019) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
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1
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Anonymous
07/23/2012
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol INHBA
Entrez
Uniprot