Recombinant Human Indian Hedgehog/Ihh Animal-Free Protein

SDS-Page: Recombinant Human Indian Hedgehog/Ihh Animal-Free Protein [NBP2-76244] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Human Indian Hedgehog/Ihh Animal-Free Protein [NBP2-76244] - Indian Hedgehog/Ihh induced alkaline phosphatase activity of CCL-226 cells.

Product Discontinued

Recombinant Human Indian Hedgehog/Ihh Animal-Free Protein Summary

• Description: For prolonged storage, dilute to working aliquots.
  
  Expiration Date:
  12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
• Specificity: Indian Hedgehog/Ihh is a monomer, 19.9 kDa (177 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IHH
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
• Theoretical MW: 19.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: 10 mM HCI at 0.1 mg/mL

Alternate Names for Recombinant Human Indian Hedgehog/Ihh Animal-Free Protein

• BDA1
• BDA1Indian hedgehog homolog (Drosophila)
• HHG2
• HHG-2
• Ihh
• Indian hedgehog (Drosophila) homolog
• Indian hedgehog homolog
• indian hedgehog protein
• Indian Hedgehog

Background

Hedgehog, WNT, FGF and BMP signaling pathways network together during embryogenesis, tissue regeneration, and carcinogenesis. Aberrant activation of Hedgehog signaling pathway leads to pathological consequences in a variety of human tumors, such as gastric cancer and pancreatic cancer (1). The hedgehog family of morphogens are regulators of cell proliferation, differentiation and cell-cell communication. These morphogens have been shown to have important roles in organogenesis, spermatogenesis, stem cell maintenance and oncogenesis. Indian hedgehog (IHH) has been shown to be expressed in the uterine epithelium under the control of the steroid hormone, progesterone (2). It has been shown that IHH, synthesized by prehypertrophic and hypertrophic chondrocytes, regulates the site of hypertrophic differentiation by signaling to the periarticular growth plate and also determines the site of bone collar formation in the adjacent perichondrium. By providing crucial local signals from prehypertrophic and hypertrophic chondrocytes to both chondrocytes and preosteoblasts, IHH couples chondrogenesis to osteogenesis in endochondral bone development (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IHH