INCENP Antibody

Western Blot: INCENP Antibody [NB100-2286] - Western blot analysis of HeLa (1), A431 (2), MCF7 (3), HEK293 (4), Neuro2a (5), and Raw 264.7 (6) cell lysate using INCENP antibody at a concentration of 2 ug/ml.

Immunocytochemistry/Immunofluorescence: INCENP Antibody [NB100-2286] - Detection of Human INCENP by Immunocytochemistry. Sample: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-INCENP (NB100-2286) used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h& l)-FITC used at a dilution of 1:100.

Immunocytochemistry/Immunofluorescence: INCENP Antibody [NB100-2286] - INCENP antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunocytochemistry/Immunofluorescence: INCENP Antibody [NB100-2286] - INCENP antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A concentration of 0.006 ug/ml was used. Image objective 40x.

Immunocytochemistry/Immunofluorescence: INCENP Antibody [NB100-2286] - Detection of Human INCENP by Immunocytochemistry. Sample: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-INCENP (NB100-2806) used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h& l)-FITC used at a dilution of 1:100.

Product Discontinued

INCENP Antibody Summary

• Immunogen: The epitope recognized by this antibody maps to a region between residue 325 and 400 of human INCENP using the numbering given in entry Q9NQS7 (GeneID 3619).
• Localization: Nucleus, Centromeres.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INCENP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.006-0.01 ug/ml
  • Western Blot 2 ug/ml
• Application Notes: This INCENP antibody is useful for Immunocytochemistry/Immunofluorescence. With formaldehyde-fixed cells permeabilization with Triton-X 100 is recommended. Likely to work with frozen sections.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for INCENP Antibody

• binds and activates aurora-B and -C in vivo and in vitro
• chromosomal passenger protein
• FLJ31633
• inner centromere protein antigens (135kD, 155kD)
• inner centromere protein antigens 135/155kDa
• inner centromere protein INCENP
• inner centromere protein
• MGC111393

Background

INCENP (inner centromere protein) is one of the key components of the CPC complex (BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC) which implicates in the regulation of mitotic cell division. CPC complex play a critical role at centromeres in facilitating correct chromosome alignment/segregation and is essential for chromatin-induced microtubule stabilization as well as spindle assembly. INCENP has been shown to localize to inner kinetochore and on chromosome arms and inner centromeres from prophase through metaphase and then transferring to spindle midzone and midbody from anaphase through cytokinesis. INCENP exists as a homodimer or heterodimer, interacts with several proteins that includes H2AFZ, CBX3, tubulin beta chain, AURKB/AURKC, EVI5, CDCA8, BIRC5, CBX5; CBX5, POGZ, and JTB. Moreover, INCENP colocalizes with AURKB at mitotic chromosomes where INCENP-AURKB/AURKC association is important for its functions and INCENP also binds directly to microtubules for regulating the kinetochore localization of BUB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INCENP
• Entrez:
  • 3619(Human)
• Uniprot:
  • Q9NQS7(Human)