Importin beta/KPNB1 Antibody (845208) [Alexa Fluor® 488]

• Applications: WB, ICC/IF
• Clone: 845208
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Importin beta/KPNB1 Antibody (845208) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Importin β/KPNB1
  Met1-Gly155
  Accession # Q14974
• Specificity: Detects human Importin beta /KPNB1 in ELISA and Western Blot. It detects mouse and rat Importin beta /KPNB1 in Western Blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

KPNB1 (Karyopherin subunit beta 1), also called Importin beta 1, NTF97 (nuclear transport factor, 97 kDa) or PTAC97 (pore targeting complex, 97 kDa), is a member of the Importin b family of proteins. It is ubiquitously expressed and plays many roles in the nucleus. Primarily, KPNB1 transports various complexes from cytoplasm into nucleus, either alone or with a KPNA adaptor. The 876 amino acid (aa) human KPNB1 contains an N-terminal IBB/Importin b domain (aa 21-101), and eight HEAT (solenoid) domains (aa 124-726). A 731 aa splice variant begins with Met146, while an 845 aa variant shows a 2 aa substitution for aa 702-734. Binding sites for adaptors and for its regulator, Ran-GTP, overlap within the HEAT domains. Human KPNB1 aa 1-155 shares 99% and 97% aa sequence identity with mouse and rat KPNB1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.