Importin beta/KPNB1 Antibody (3E9)

Product Discontinued

Importin beta/KPNB1 Antibody (3E9) Summary

• Immunogen: Purified NTF97 from bovine erythrocytes.
• Localization: Nuclear and Nuclear membrane
• Specificity: Detects nuclear transport factor p97 (NTF 97).This does not detect bacterially expressed recombinant NTF 97.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: KPNB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Flow Cytometry 1 ug
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Inhibition Activity
  • Simple Western 1:100
  • Western Blot 1:5000
• Application Notes: ChIP usage was reproted in scientific literature. May be useful in Flow. WB: Detects an approx. 97 kDa protein representing NTF 97 in MDBK cell homogenate. IF: Staining of NTF 97 in MDBK cells results in the labeling of both the nuclear envelope (NE) and the cytoplasm and shows discontinuous, punctate staining along the rim of the NE.This antibody can inhibit import of nuclear localization sequence-containing proteins in permeabilized cells.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 7615630). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Importin beta/KPNB1 Antibody (3E9)

• IMB1
• Impnb
• Importin 1
• importin 90
• Importin beta
• importin beta-1 subunit
• importin subunit beta-1
• importin-90
• IPO1
• IPOB
• karyopherin (importin) beta 1
• Karyopherin subunit beta-1
• KPNB1
• MGC2155
• MGC2156
• MGC2157
• NTF97
• NTF97Pore targeting complex 97 kDa subunit
• Nuclear factor p97
• PTAC97

Background

The accumulation of proteins in the nucleus is mediated by short sequences of basic amino acids called nuclear localization sequences (NLSs). These sequences are necessary and sufficient to direct a protein or inert carrier to the nuclear interior. Nuclear protein import is accomplished by two sequential, energy dependent events. The first step, docking at the nuclear pore complex, requires a 54/56 kDa nuclear localization signal receptor (a-karyopherin, importin-a, SRP-a) and the nuclear transport factor p97 (NTF 97, b-karyopherin, importin-b). The second step, translocation across the nuclear envelope (NE), requires the GTP-binding protein, Ran/TC4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Importin beta/KPNB1 Antibody (NB120-2811)(4)

Publications using NB120-2811

• Fang W, Deng Z, Benadjaoud F et al. Cathepsin B deficiency ameliorates liver lipid deposition, inflammatory cell infiltration, and fibrosis after diet-induced nonalcoholic steatohepatitis Transl Res 2020-05-11 [PMID: 32434697] (WB, Mouse)
• Arnold M, Nath A, Hauber J et al. Multiple importins function as nuclear transport receptors for the Rev protein of human immunodeficiency virus type 1. J Biol Chem. 2006-07-01 [PMID: 16704975]
• Wamhoff BR, Bowles DK, Dietz NJ et al. Exercise training attenuates coronary smooth muscle phenotypic modulation and nuclear Ca2+ signaling. Am J Physiol Heart Circ Physiol. 2002-12-01 [PMID: 12388302]
• Chi NC, Adam EJ, Adam SA et al. Sequence and characterization of cytoplasmic nuclear protein import factor p97. J Cell Biol. 1995-07-01 [PMID: 7615630]

Bioinformatics

• Gene Symbol: KPNB1
• Entrez:
  • 3837(Human)
  • 24917(Rat)