RanBP1 Antibody

Western Blot: RanBP1 Antibody [NB100-1443] - Staining of A431 (A) with antibody at 0.03 ug/mL, HeLa (B) with antibody at 0.5 ug/mL and MCF7 (C) cell lysate with antibody at 0.1 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NB100-1443] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: RanBP1 Antibody [NB100-1443] - Staining of paraffin embedded Human Kidney with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: RanBP1 Antibody [NB100-1443] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: RanBP1 Antibody [NB100-1443] - Staining of Human Duodenum lysate with antibody at 0.3 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NB100-1443] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RanBP1 Antibody Summary

• Description: Novus Biologicals Goat RanBP1 Antibody (NB100-1443) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-RanBP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-LSVKEETKEDAEEKQ corresponding to C-Terminus according to NP_002873.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RANBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.03 - 0.5 ug/mL
• Control:

  RanBP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RanBP1 Antibody

• HTF9A
• MGC88701
• RAN binding protein 1
• Ran-binding protein 1
• RanBP1
• ran-specific GTPase-activating protein

Background

Transport of macromolecules into and out of the nucleus occurs through nuclear pore complexes (NPC). This energy-dependent process involves nucleoporins at the NPC and associated transport proteins and GTP. Ran binding protein 1 (RanBP 1) belongs to a family of proteins that bind Ran GTPase which is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RanBP1 Antibody (NB100-1443)(1)

Publication using NB100-1443

• Bischoff FR, Krebber H, Smirnova E et al. Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1. EMBO J 1995-02-15 [PMID: 7882974]

Bioinformatics

• Gene Symbol: RANBP1
• Entrez:
  • 5902(Human)
• Uniprot:
  • NP_002873.(Human)