Importin alpha 3/KPNA4 Antibody

Western Blot: Importin alpha 3/KPNA4 Antibody [NB100-81651] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-81651 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.

Immunohistochemistry-Paraffin: Importin alpha 3/KPNA4 Antibody [NB100-81651] - Human prostate carcinoma. Antibody used at a dilution of 1:200.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Importin alpha 3/KPNA4 Antibody Summary

• Description: Novus Biologicals Rabbit Importin alpha 3/KPNA4 Antibody (NB100-81651) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-Importin alpha 3/KPNA4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 471 and 521 of human karyopherin alpha 4 using the numbering given in entry NP_002259.1 (GeneID 3840).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KPNA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  Importin alpha 3/KPNA4 Overexpression Lysate

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32320654).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Importin alpha 3/KPNA4 Antibody

• Importin alpha 3
• Importin alpha Q1
• importin subunit alpha-4
• importin-alpha-Q1
• IPOA3
• karyopherin alpha 4 (importin alpha 3)
• KPNA4
• MGC12217
• MGC26703
• Qip1
• QIP12
• QIP1Karyopherin subunit alpha-4
• SRP3

Background

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Importin alpha 3/KPNA4 Antibody (NB100-81651)(1)

Publication using NB100-81651

• Thiele S, Stanelle-Bertram S et al. Cellular Importin-alpha 3 Expression Dynamics in the Lung Regulate Antiviral Response Pathways against Influenza A Virus Infection. Cell Rep 2020-04-21 [PMID: 32320654] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: KPNA4
• Entrez:
  • 3840(Human)
• Uniprot:
  • NP_002259.1(Human)