Importin-13 Antibody

Product Discontinued

Importin-13 Antibody Summary

• Immunogen: Synthetic peptide: CRGLHGTDYTADY, representing the C-terminus of the human protein (residues 951-963), according to NP_055467.
• Localization: Cytoplasmic and Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IPO13
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:32000
• Application Notes: This antibody can be used for Western Blot and ELISA, In Western Blot, preliminary experiments gave an approx 50kDa and 40kDa bands in Hela and Human Brain lysates. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 110kDda according to NP_055467. Not tested in other applications. Recommended Starting Dilution:* ELISA (peptide): 1:16,000 WB: 1ug/ml *Optimal dilutions should be determined by the end user.

Reactivity Notes

Cross reacts with Human and Mouse. Expected from sequence similarity: Rat, Dog and Cow.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Importin-13 Antibody

• IMP13
• importin 13
• importin-13
• KAP13
• karyopherin 13
• Karyopherin-13
• KIAA0724karyopherin-13
• LGL2
• Ran binding protein 13
• Ran-binding protein 13
• RanBP13
• RANBP13late gestation lung 2

Background

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13. Expressed in fetal brain, heart, intestine and kidney. Belongs to the importin beta family. Contains 14 HEAT repeats. Contains 1 importin N-terminal domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IPO13
• Entrez:
  • 9670(Human)
• Uniprot:
  • NP_055467.(Human)