Recombinant Human IMPDH1 Protein

Product Discontinued

Recombinant Human IMPDH1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-514 of Human IMPDH1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MADYLISGGT GYVPEDGLTA QQLFASADGL TYNDFLILPG FIDFIADEVD LTSALTRKIT LKTPLISSPM DTVTEADMAI AMALMGGIGF IHHNCTPEFQ ANEVRKVKKF EQGFITDPVV LSPSHTVGDV LEAKMRHGFS GIPITETGTM GSKLVGIVTS RDIDFLAEKD HTTLLSEVMT PRIELVVAPA GVTLKEANEI LQRSKKGKLP IVNDCDELVA IIARTDLKKN RDYPLASKDS QKQLLCGAAV GTREDDKYRL DLLTQAGVDV IVLDSSQGNS VYQIAMVHYI KQKYPHLQVI GGNVVTAAQA KNLIDAGVDG LRVGMGCGSI CITQEVMACG RPQGTAVYKV AEYARRFGVP IIADGGIQTV GHVVKALALG ASTVMMGSLL AATTEAPGEY FFSDGVRLKK YRGMGSLDAM EKSSSSQKRY FSEGDKVKIA QGVSGSIQDK GSIQKFVPYL IAGIQHGCQD IGARSLSVLR SMMYSGELKF EKRTMSAQIE GGVHGLHSYE KRLY

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IMPDH1
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 57.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human IMPDH1 Protein

• DKFZp781N0678
• EC 1.1.1.205
• IMP (inosine 5'-monophosphate) dehydrogenase 1
• IMP (inosine monophosphate) dehydrogenase 1
• IMP Dehydrogenase 1
• IMPD 1
• IMPD
• IMPD1
• IMPDH 1
• IMPDH1
• IMPDH-I
• inosine-5'-monophosphate dehydrogenase 1
• LCA11
• LCA11RP10
• retinitis pigmentosa 10 (autosomal dominant)
• RP10
• sWSS2608

Background

Inosine-5'-monophosphate dehydrogenase 1, also known as IMPDH1 catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). Also, IMPDH1 is a ubiquitously expressed enzyme, functioning as a homotetramer, which catalyzed the rate-limiting step in de novo synthesis of guanine nucleotides. As such, it plays an important role in cyclic nucleoside metabolism within photoreceptors. Defects in the IMPDH1 gene are a cause of retinitis pigmentosa type 10. Recombinant human IMPDH1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for IMPDH1 Recombinant Protein (NBP1-45252). (Showing 1 - 1 of 1 FAQ).

1. Do you have a kit for measuring IMPDH activity?
   • We currently do not have any kits to measure IMPDH activity, however we have a number of IMPDH antibody pairs that can be used for WB/IP and ELISA.

Bioinformatics

• Gene Symbol: IMPDH1