ILVBL Antibody (OTI8B12) - Azide and BSA Free

Western Blot: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILVBL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ILVBL.

Immunocytochemistry/Immunofluorescence: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ILVBL.

Immunohistochemistry: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of paraffin-embedded Human breast tissue using anti-ILVBL mouse monoclonal antibody.

Western Blot: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ILVBL monoclonal antibody.

Immunohistochemistry: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ILVBL mouse monoclonal antibody.

Immunohistochemistry: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ILVBL mouse monoclonal antibody.

Immunohistochemistry: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ILVBL mouse monoclonal antibody.

Immunohistochemistry: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of paraffin-embedded Human prostate tissue using anti-ILVBL mouse monoclonal antibody.

CyTOF-ready: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Analysis of Hela cells, using anti-ILVBL antibody, (Red), compared to a nonspecific negative control antibody (Blue).

CyTOF-ready: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Analysis of Jurkat cells, using anti-ILVBL antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI8B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ILVBL Antibody (OTI8B12) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ILVBL Antibody (OTI8B12) - Azide and BSA Free (NBP2-00883) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ILVBL(NP_006835) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ILVBL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 67.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ILVBL Antibody (OTI8B12) - Azide and BSA Free

• acetolactate synthase homolog
• acetolactate synthase-like protein
• AHASFLJ39061,209L8
• EC 2.2.1
• EC 2.2.1.-
• ILV2H
• ilvB (bacterial acetolactate synthase)-like
• IlvB-like protein
• MGC1269
• MGC19535

Background

The protein encoded by the ILVBL gene shares similarity with several thiamine pyrophosphate-binding proteins identified inbacteria, yeast, and plants. The highest degree of similarity is found with bacterial acetolactate synthases (AHAS),which are enzymes that catalyze the first step in branched-chain amino acid biosynthesis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ILVBL