ILVBL Antibody (OTI8B12) - Azide and BSA Free

Images

 
Western Blot: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILVBL (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ILVBL.
Immunohistochemistry: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of paraffin-embedded Human breast tissue using anti-ILVBL mouse monoclonal antibody.
Western Blot: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ILVBL monoclonal antibody.
Immunohistochemistry: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ILVBL mouse monoclonal antibody.
Immunohistochemistry: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ILVBL mouse monoclonal antibody.
Immunohistochemistry: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ILVBL mouse monoclonal antibody.
Immunohistochemistry: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Staining of paraffin-embedded Human prostate tissue using anti-ILVBL mouse monoclonal antibody.
CyTOF-ready: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Analysis of Hela cells, using anti-ILVBL antibody, (Red), compared to a nonspecific negative control antibody (Blue).
CyTOF-ready: ILVBL Antibody (OTI8B12) - Azide and BSA Free [NBP2-71661] - Analysis of Jurkat cells, using anti-ILVBL antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI8B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

ILVBL Antibody (OTI8B12) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ILVBL(NP_006835) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ILVBL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:500-2000
Theoretical MW
67.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for ILVBL Antibody (OTI8B12) - Azide and BSA Free

  • acetolactate synthase homolog
  • acetolactate synthase-like protein
  • AHASFLJ39061,209L8
  • EC 2.2.1
  • EC 2.2.1.-
  • ILV2H
  • ilvB (bacterial acetolactate synthase)-like
  • IlvB-like protein
  • MGC1269
  • MGC19535

Background

The protein encoded by the ILVBL gene shares similarity with several thiamine pyrophosphate-binding proteins identified inbacteria, yeast, and plants. The highest degree of similarity is found with bacterial acetolactate synthases (AHAS),which are enzymes that catalyze the first step in branched-chain amino acid biosynthesis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ILVBL