| Reactivity | HuSpecies Glossary |
| Applications | Flow |
| Clone | 656688 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Alexa Fluor 594 |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB6287U-100UG | |||
| Alexa Fluor 405 | FAB6287V-100UG | |||
| Alexa Fluor 488 | FAB6287G-100UG | |||
| Alexa Fluor 647 | FAB6287R-100UG | |||
| Alexa Fluor 700 | FAB6287N-100UG | |||
| Alexa Fluor 750 | FAB6287S-100UG | |||
| Unconjugated | MAB6287 | |||
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human LILRA4/CD85g/ILT7 Glu24-Asn446 Accession # P59901 |
| Specificity | Detects human LILRA4/CD85g/ILT7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) LILRA5, rhILT2, 3, 4, 5, or 6 is observed. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LILRA4 |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
| Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| Buffer | Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
| Preservative | 0.09% Sodium Azide |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
LILRA4, also known as ILT7 and CD85g, is a 499 amino acid (aa) type I transmembrane glycoprotein that contains four Ig-like domains in its extracellular region. LILRA4 is selectively expressed on plasmacytoid dendritic cells (pDC). It binds to BST2/CD317 on bone marrow stromal cells and associates with the gamma subunit of Fc epsilon RI. This receptor complex transmits signals that inhibit the TLR-induced production of type I interferon. The LILRA4-mediated interactions between pDC and tumor cells similarly inhibit the production of proinflammatory cytokines and contribute to reduced anti-tumor immune responses. Alternate splicing of human LILRA4 generates an isoform that lacks the N-terminal 43 aa of the extracellular domain.
![GITR/TNFRSF18 Antibody (110416) [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "GITR/TNFRSF18 Antibody (110416) [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | FAB6287U | |
| Alexa Fluor 405 | FAB6287V | |
| Alexa Fluor 488 | FAB6287G | |
| Alexa Fluor 594 | FAB6287T | |
| Alexa Fluor 647 | FAB6287R | |
| Alexa Fluor 700 | FAB6287N | |
| Alexa Fluor 750 | FAB6287S |
Diseases for ILT7/CD85g/LILRA4 Antibody (FAB6287T)Discover more about diseases related to ILT7/CD85g/LILRA4 Antibody (FAB6287T).
| Pathways for ILT7/CD85g/LILRA4 Antibody (FAB6287T)View related products by pathway.
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PTMs for ILT7/CD85g/LILRA4 Antibody (FAB6287T)Learn more about PTMs related to ILT7/CD85g/LILRA4 Antibody (FAB6287T).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: BST2 Antibody (120G8.04) [DDX0390P-100] - Murine peyer patch: BST-2](http://images.novusbio.com/images2/BST2-Antibody-(120G8.04)-Immunocytochemistry-Immunofluorescence-DDX0390P-100-img0005.jpg)
![Immunohistochemistry: BST2 Antibody (120G8.04) [DDX0390P-100] - IHC staining of murine spleen cryosections](http://images.novusbio.com/images2/BST2-Antibody-(120G8.04)-Immunohistochemistry-DDX0390P-100-img0006.jpg)
![Flow Cytometry: BST2 Antibody (120G8.04) [DDX0390P-100] - In vivo depletion of mouse PDCs (gated on CD11c+CD3- cells).](http://images.novusbio.com/images2/BST2-Antibody-(120G8.04)-Flow-Cytometry-DDX0390P-100-img0008.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody.](http://images.novusbio.com/images2/TLR9-Antibody-(26C593.2)-Simple-Western-NBP2-24729-img0029.jpg)
![Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 protein on epithelial cells. HNEC, Detroit-562 and FaDu were stained intracellularly with PE-Ab against TLR9 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0098239), licensed under a CC-BY licence.](http://images.novusbio.com/images2/TLR9-Antibody-(26C593.2)-Flow-Cytometry-NBP2-24729-img0025.jpg)
![Knockdown Validated: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY licence.](http://images.novusbio.com/images2/TLR9-Antibody-(26C593.2)-Knockdown-Validated-NBP2-24729-img0031.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Immunohistochemistry: TLR7 Antibody [NBP2-24906] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY licence.](http://images.novusbio.com/images2/TLR7-Antibody-Immunohistochemistry-NBP2-24906-img0022.jpg)
![Flow Cytometry: TLR7 Antibody [NBP2-24906] - An intracellular stain was performed on THP-1 cells with NBP2-24906 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/images2/TLR7-Antibody-Flow-Cytometry-NBP2-24906-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: TLR7 Antibody [NBP2-24906] - Immunolocalization of TLR7 (A) and glial fibrillary acidic protein (GFAP) (B) in murine myenteric plexus. Most of the GFAP positive cells were also positive for TLR7 in the merged image (C, yellow staining). (Courtesy of Barajon I, et al., Journal of Histochemistry and Cytochemistry, 57(11): 1013-;1023, 2009.)](http://images.novusbio.com/images2/TLR7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24906-img0002.jpg)
![Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.](http://images.novusbio.com/images2/IFN-alpha-1-Antibody-Western-Blot-NBP2-75930-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/IFN-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75930-img0004.jpg)
![Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/IFN-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-75930-img0007.jpg)
![Western Blot: Phosducin Antibody [NBP2-94660] - Analysis of extracts of various cell lines, using Phosducin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/images2/Phosducin Antibody-Western Blot-NBP2-94660-img0003.jpg)
![Immunohistochemistry-Paraffin: Phosducin Antibody [NBP2-94660] - Paraffin-embedded rat retina using Phosducin.](http://images.novusbio.com/images2/Phosducin-Antibody-Immunohistochemistry-Paraffin-NBP2-94660-img0002.jpg)
![Immunohistochemistry-Paraffin: Phosducin Antibody [NBP2-94660] - Paraffin-embedded mouse retina using Phosducin.](http://images.novusbio.com/images2/Phosducin-Antibody-Immunohistochemistry-Paraffin-NBP2-94660-img0001.jpg)
![Western Blot: LRRC25 Antibody (1D9) [NBP2-03747] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC25.](http://images.novusbio.com/images/LRRC25 Antibody (1D9)-Western Blot-NBP2-03747-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: LRRC25 Antibody (1D9) [NBP2-03747] Staining of COS7 cells transiently transfected by pCMV6-ENTRY LRRC25.](http://images.novusbio.com/images/LRRC25 Antibody (1D9)-Immunocytochemistry-Immunofluorescence-NBP2-03747-img0006.jpg)
![Immunohistochemistry-Paraffin: LRRC25 Antibody (1D9) [NBP2-03747] - Staining of paraffin-embedded Human lymphoma tissue using anti-LRRC25 mouse monoclonal antibody.](http://images.novusbio.com/images/LRRC25 Antibody (1D9)-Immunohistochemistry-Paraffin-NBP2-03747-img0005.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/images2/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/images2/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19371-img0005.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0014.jpg)
![Western Blot: Spi-B Antibody (4G5) [NBP2-37458] - Analysis using SPIB mouse mAb against A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate.](http://images.novusbio.com/images2/Spi-B-Antibody-(4G5)-Western-Blot-NBP2-37458-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/Spi-B-Antibody-(4G5)-Immunocytochemistry-Immunofluorescence-NBP2-37458-img0004.jpg)
![Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of paraffin-embedded esophageal cancerwith DAB staining.](http://images.novusbio.com/images2/Spi-B-Antibody-(4G5)-Immunohistochemistry-NBP2-37458-img0007.jpg)
![Western Blot: PNKD Antibody [NBP1-88347] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PNKD antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/images2/PNKD Antibody-Western Blot-NBP1-88347-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PNKD Antibody [NBP1-88347] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/images2/PNKD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88347-img0006.jpg)
![Immunohistochemistry-Paraffin: PNKD Antibody [NBP1-88347] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/images2/PNKD-Antibody-Immunohistochemistry-Paraffin-NBP1-88347-img0005.jpg)
![Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Western-Blot-NBP1-92684-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0003.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/TOR-mTOR [p Ser2448] Antibody-Western Blot-NB600-607-img0006.jpg)
![Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.](http://images.novusbio.com/images2/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Detection of a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/images2/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0004.jpg)