ILT6/CD85e/LILRA3 Antibody [Alexa Fluor® 488]

Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Goat Anti-Human LILRA3/CD85e Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2574G) or (B) Normal Goat IgG Alexa Fluor 488 Control (Catalog # IC108G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

ILT6/CD85e/LILRA3 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ILT6/CD85e
  Arg18-Glu439
  Accession # Q8N6C8
• Specificity: Detects human ILT6/CD85e in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) ILT2, rhILT4, ILT5 is observed, and less than 5% cross-reactivity with rhLIR5 and rhLIR7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LILRA3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT6/CD85e/LILRA3 Antibody [Alexa Fluor® 488]

• CD85 antigen-like family member E
• CD85e antigen
• CD85e
• HM31
• HM43
• ILT6
• ILT-6
• ILT6CD85e
• Immunoglobulin-like transcript 6
• leucocyte immunoglobulin-like receptor
• Leukocyte immunoglobulin-like receptor 4
• leukocyte immunoglobulin-like receptor A3
• leukocyte immunoglobulin-like receptor subfamily A member 3
• leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member3
• LILRA3
• LIR4
• LIR4CD85E
• LIR-4HM43e3
• Monocyte inhibitory receptor HM43/HM31

Background

Human LIR-4 (Leukocyte Ig-like receptor #4; also ILT6 and LILRA3) is an Ig superfamily member that belongs to the leukocyte receptor complex/cluster. Mature LIR-4 is 416 amino acids in length and contains four C2-type Ig-like domains. Unlike other LIR family members, LIR-4 is actively secreted. At least one alternate splice form is known which shows a 64 amino acid deletion in the C-terminal half of the second Ig-like domain. Multiple alleles exist in human, and 5-10% of the population have a defective gene which produces no LIR-4 product. Rodent LIR-4 has not been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ILT6/CD85e/LILRA3 Antibody (IC2574G)(1)

Publication using IC2574G

• Hong Yu, Hui Liu, Yang Zhao, Huaquan Wang, Chunyan Liu, Weiwei Qi et al. Upregulated expression of leukocyte immunoglobulin‑like receptor A3 in patients with severe aplastic anemia Experimental and Therapeutic Medicine 2021-02-11 [PMID: 33732319]

Bioinformatics

• Gene Symbol: LILRA3
• Uniprot:
  • Q8N6C8()