ILT4/CD85d/LILRB2 Overexpression Lysate (Native)

Images

 
Western Blot: LILRB2 Overexpression Lysate (Adult Normal) [NBL1-12527] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LILRB2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ILT4/CD85d/LILRB2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207770 and based on accession number NM_005874. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2 (LILRB2), transcript variant 1, mRNA.
Gene
LILRB2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
64.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ILT4/CD85d/LILRB2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ILT4/CD85d/LILRB2 Overexpression Lysate (Native)

  • CD85 antigen-like family member D
  • CD85d antigen
  • CD85d
  • Ig-like transcript 4
  • ILT4
  • ILT-4
  • ILT4CD85d
  • Immunoglobulin-like transcript 4
  • Leukocyte immunoglobulin-like receptor 2
  • leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform
  • leukocyte immunoglobulin-like receptor subfamily B member 2
  • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
  • LILRB2
  • LIR2
  • LIR2CD85D
  • LIR-2subfamily A (with TM domain), member 6
  • member 2
  • MIR10
  • MIR10LILRA6
  • Monocyte/macrophage immunoglobulin-like receptor 10

Background

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB20172
Species: Hu
Applications: WB, CyTOF-reported, Neut
AF2425
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP1-43123
Species: Hu, Mu
Applications: WB, B/N, ELISA, Flow, ICC/IF, CyTOF-ready
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
MAB6364
Species: Hu
Applications: Flow, CyTOF-ready
NB120-6405
Species: Rt
Applications: B/N, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
MAB1806
Species: Hu
Applications: WB, Flow, CyTOF-ready
H00003126-M01
Species: Hu
Applications: WB, ELISA, IHC-P
MAB140
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
AF4885
Species: Mu
Applications: WB, IP
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-25208
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
MAB12251
Species: Hu
Applications: Flow, CyTOF-ready

Publications for ILT4/CD85d/LILRB2 Lysate (NBL1-12527) (0)

There are no publications for ILT4/CD85d/LILRB2 Lysate (NBL1-12527).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ILT4/CD85d/LILRB2 Lysate (NBL1-12527) (0)

There are no reviews for ILT4/CD85d/LILRB2 Lysate (NBL1-12527). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ILT4/CD85d/LILRB2 Lysate (NBL1-12527) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ILT4/CD85d/LILRB2 Products

Bioinformatics Tool for ILT4/CD85d/LILRB2 Lysate (NBL1-12527)

Discover related pathways, diseases and genes to ILT4/CD85d/LILRB2 Lysate (NBL1-12527). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ILT4/CD85d/LILRB2 Lysate (NBL1-12527)

Discover more about diseases related to ILT4/CD85d/LILRB2 Lysate (NBL1-12527).
 

Pathways for ILT4/CD85d/LILRB2 Lysate (NBL1-12527)

View related products by pathway.

PTMs for ILT4/CD85d/LILRB2 Lysate (NBL1-12527)

Learn more about PTMs related to ILT4/CD85d/LILRB2 Lysate (NBL1-12527).

Blogs on ILT4/CD85d/LILRB2

There are no specific blogs for ILT4/CD85d/LILRB2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

CD4 Antibody
NBP1-19371

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ILT4/CD85d/LILRB2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol LILRB2
Entrez