Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence) Summary
| Description |
Assay Length: 3 h 30 min |
| Specificity |
This kit recognizes HumanI LT4/CD85d/LILRB2 in samples. No significant cross-reactivity or interference between Human LILRB2 and analogues was observed |
| Standard Curve Range |
31.25 - 2000 pg/mL |
| Sensitivity |
18.75 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 10 |
| Intra-Assay |
CV% < 10 |
| Spike Recovery |
80 - 120% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Chemiluminescence) |
| Gene |
LILRB2 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated Detection Ab Diluent
- Certificate of Analysis
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate(100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate (Dismountable)
- Plate Sealer
- Product Description
- Reference Standard & Sample Diluent
- Reference Standard
- Substrate Reagent A
- Substrate Reagent B
|
Alternate Names for Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence)
Background
LILRB2, also known as Leukocyte immunoglobulin-like receptor subfamily B member 2, has 2 isoforms, a 598 amino acid isoform that is 65 kDa and a 597 amino acid isoform that is approx. 65 kDa; expressed on monocytes and B-cells, and at lower levels on dendritic cells and in natural killer (NK) cells; as a receptor binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response; also competes with CD8A for binding to class I MHC antigens and inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. Disease research is currently being performed with relation to LILRB2 and herpes simplex, chronic lymphocytic leukemia, lymphocytic leukemia, and leukemia. Interactions with this protein have been shown to involve PTPN6, FCGR1A, HLA-F, HLA-G, CD1D and other proteins in osteoclast differentiation, immune-regulatory interactions between a lymphoid and a non-lymphoid cell, adaptive immune system, and immune system pathways.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for ILT4/CD85d/LILRB2 ELISA Kit (NBP3-49817). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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