Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence)

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Standard Curve Reference.
Samples were spiked with high concentrations of Human ILT4/CD85d/LILRB2, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates, and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence) Summary

Description
Assay Length: 3 h 30 min
Specificity
This kit recognizes HumanI LT4/CD85d/LILRB2 in samples. No significant cross-reactivity or interference between Human LILRB2 and analogues was observed
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10
Intra-Assay
CV% < 10
Spike Recovery
80 - 120%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
LILRB2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate(100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro CLIA Plate (Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Substrate Reagent A
  13. Substrate Reagent B

Alternate Names for Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence)

  • CD85 antigen-like family member D
  • CD85d antigen
  • CD85d
  • Ig-like transcript 4
  • ILT4
  • ILT-4
  • ILT4CD85d
  • Immunoglobulin-like transcript 4
  • Leukocyte immunoglobulin-like receptor 2
  • leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform
  • leukocyte immunoglobulin-like receptor subfamily B member 2
  • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
  • LILRB2
  • LIR2
  • LIR2CD85D
  • LIR-2subfamily A (with TM domain), member 6
  • member 2
  • MIR10
  • MIR10LILRA6
  • Monocyte/macrophage immunoglobulin-like receptor 10

Background

LILRB2, also known as Leukocyte immunoglobulin-like receptor subfamily B member 2, has 2 isoforms, a 598 amino acid isoform that is 65 kDa and a 597 amino acid isoform that is approx. 65 kDa; expressed on monocytes and B-cells, and at lower levels on dendritic cells and in natural killer (NK) cells; as a receptor binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response; also competes with CD8A for binding to class I MHC antigens and inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. Disease research is currently being performed with relation to LILRB2 and herpes simplex, chronic lymphocytic leukemia, lymphocytic leukemia, and leukemia. Interactions with this protein have been shown to involve PTPN6, FCGR1A, HLA-F, HLA-G, CD1D and other proteins in osteoclast differentiation, immune-regulatory interactions between a lymphoid and a non-lymphoid cell, adaptive immune system, and immune system pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for ILT4/CD85d/LILRB2 ELISA Kit (NBP3-49817). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol LILRB2