ILT3/CD85k/LILRB4 Antibody (293623) [Fluorescein]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL-3 Ra/CD123 APC-conjugated Monoclonal Antibody (Catalog # FAB301A) and either (A) Mouse Anti-Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB24251F) or (B) Mouse IgG2AFluorescein Isotype Control (Catalog # IC003F). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 293623
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

ILT3/CD85k/LILRB4 Antibody (293623) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ILT3/CD85k
  Pro17-His257
  Accession # ABM86208
• Specificity: Detects human ILT3/CD85k in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with with recombinant human (rh) ILT5 is observed. No cross-reactivity with rhILT1, rhILT2, rhILT4, rhILT7, rhILT11, rhLIR-6 or rhLIR-8 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LILRB4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT3/CD85k/LILRB4 Antibody (293623) [Fluorescein]

• CD85 antigen-like family member K
• CD85k antigen
• CD85k
• HM18
• ILT3
• ILT-3
• ILT3CD85K
• leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
• LILRB4
• LIR5
• LIR5LILRB5
• LIR-5subfamily B, member 4
• member 4

Background

Immunoglobulin-Like Transcript 3 (ILT3), also known as Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4) and Leukocyte Immunoglobulin-like Receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ILT3/CD85k/LILRB4 Antibody (FAB24251F)(3)

Publications using FAB24251F

• Ulges A, Klein M, Reuter S, Gerlitzki B, Hoffmann M, Grebe N, Staudt V, Stergiou N, Bohn T, Bruhl T, Muth S, Yurugi H, Rajalingam K, Bellinghausen I, Tuettenberg A, Hahn S, Reissig S, Haben I, Zipp F, Waisman A, Probst H, Beilhack A, Buchou T, Filhol-Cochet O, Boldyreff B, Breloer M, Jonuleit H, Schild H, Schmitt E, Bopp T Protein kinase CK2 enables regulatory T cells to suppress excessive TH2 responses in vivo. Nat Immunol, 2015-01-19;16(3):267-75. 2015-01-19 [PMID: 25599562] (Flow Cytometry, Human)
• Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells. PLoS ONE, 2011-07-25;6(7):e22504. 2011-07-25 [PMID: 21799877] (Flow Cytometry, Human)
• Svajger U, Obermajer N, Anderluh M, Kos J, Jeras M DC-SIGN ligation greatly affects dendritic cell differentiation from monocytes compromising their normal function. J. Leukoc. Biol., 2011-03-29;89(6):893-905. 2011-03-29 [PMID: 21447648] (Flow Cytometry, Human)

Reviews for ILT3/CD85k/LILRB4 Antibody (FAB24251F) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/22/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 23039892
• Species: Human

reviewed by: Verified Customer Flow Human 01/22/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 23039892
• Species: Human

Bioinformatics

• Gene Symbol: LILRB4
• Uniprot:
  • ABM86208()