ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free

Flow Cytometry: ILT2/CD85j/LILRB1 Antibody (HP-F1) [NBP1-42998] - Analysis using the Biotin conjugate of NBP1-42998. Staining of normal human peripheral blood cells with 0.125 micrograms of Mouse IgG1 k Isotype Control Biotin (blue histogram) or Anti-Human CD85j (ILT2) Biotin (purple histogram) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.

Flow Cytometry: ILT2/CD85j/LILRB1 Antibody (HP-F1) [NBP1-42998] - Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (blue histogram) or Anti-Human CD85j (ILT2) APC (purple histogram). Cells in the monocyte gate were used for analysis.

• Reactivity: Hu
• Applications: Flow, Func, CyTOF-ready
• Clone: HP-F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free Summary

• Additional Information: Clone HP-F1 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody was CD85j.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LILRB1
• Purity: Protein A or G purified
• Endotoxin Note: < 0.001 ng/ug.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Functional
• Application Notes: This low endotoxin and azide free HP-F1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 uL (0.25 ug) per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells per test.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free

• CD85 antigen-like family member J
• CD85j antigen
• CD85j
• Ig-like transcript 2
• ILT2
• ILT-2
• ILT2FLJ37515
• Immunoglobulin-like transcript 2
• leukocyte Ig-like receptor-1
• Leukocyte immunoglobulin-like receptor 1
• leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform
• leukocyte immunoglobulin-like receptor subfamily B member 1
• leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
• LILRB1
• LIR1
• LIR1LIR-1MIR-7CD85MIR7CD85 antigen
• member 1
• MIR7
• Monocyte/macrophage immunoglobulin-like receptor 7

Background

The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT (immunoglobulin-like transcript)/LIR (leukocyte Ig-like receptor)/MIR (monocyte Ig-like receptor) family. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1. Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells including, subsets of NK, T and B cells. Expression has been correlated with leukemias such as ALL and CLL. Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV. The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F. The monoclonal antibody HP-F1 has been shown to reduce the amount of CD16- dependent cytolytic activity of functional NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LILRB1