ILT11/LILRA5 Overexpression Lysate

Western Blot: LILRA5 Overexpression Lysate (Adult Normal) [NBP2-06535] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LILRA5.

• Reactivity: Hu
• Applications: WB

ILT11/LILRA5 Overexpression Lysate Summary

• Description:

  ILT11/LILRA5 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC212310
  
  Accession#: NM_021250
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: LILRA5

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 32.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for ILT11/LILRA5 Overexpression Lysate

• CD85 antigen-like family member F
• CD85
• CD85f antigen
• CD85f
• ILT11
• ILT-11
• ILT11CD85f
• Immunoglobulin-like transcript 11
• leukocyte Ig-like receptor 9
• Leukocyte immunoglobulin-like receptor 9
• leukocyte immunoglobulin-like receptor subfamily A member 5 soluble
• leukocyte immunoglobulin-like receptor subfamily A member 5
• leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5
• leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
• LILRA5
• LILRB7
• LIR9
• LIR-9
• LIR9immunoglobulin-like transcript 11 protein
• member 7

Background

The protein encoded by the LILRA5 gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family. LIR family members are known to have activating and inibitory functions in leukocytes. Crosslink of this receptor protein on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses. This gene is one of the leukocyte receptor genes that form a gene cluster on the chromosomal region 19q13.4. Four alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: LILRA5