ILKAP/PP2C delta Antibody - BSA Free

Western Blot: ILKAP/PP2C delta Antibody [NBP1-85117] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Immunocytochemistry/Immunofluorescence: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human cell line A-431 shows localization to nucleus.

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Western Blot: ILKAP/PP2C delta Antibody [NBP1-85117] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human stomach shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human colon shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human endometrium shows strong nuclear positivity in glandular cells.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ILKAP/PP2C delta Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ILKAP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  ILKAP/PP2C delta Protein (NBP1-85117PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ILKAP/PP2C delta Antibody - BSA Free

• DKFZp434J2031
• EC 3.1.3.16
• FLJ10181
• ILKAP
• integrin-linked kinase-associated serine/threonine phosphatase 2C
• integrin-linked kinase-associated serine/threonine phosphatase
• MGC4846
• PP2C delta
• PP2C-DELTA
• protein phosphatase 2c, delta isozyme

Background

ILKAP is encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ILKAP