ILK Antibody (6A5) - BSA Free

Immunoprecipitation: ILK Antibody (6A5) [NBP3-26198] - Immunoprecipitating ILK in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26198 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26198 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).

Western Blot: ILK Antibody (6A5) [NBP3-26198] - Positive Western Blot detected in: Hela whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue All lanes: ILK Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 45, 37 kDa Observed band size: 51 kDa

Immunocytochemistry/Immunofluorescence: ILK Antibody (6A5) [NBP3-26198] - Staining of MCF7 Cells with ILK Antibody (6A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 6A5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ILK Antibody (6A5) - BSA Free Summary

• Description: Novus Biologicals Rabbit ILK Antibody (6A5) - BSA Free (NBP3-26198) is a recombinant monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human ILK [UniProt Q13418]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ILK
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ILK Antibody (6A5) - BSA Free

• 59 kDa serine/threonine-protein kinase
• DKFZp686F1765
• EC 2.7.11.1
• ESTM24
• ILK
• ILK1
• ILK-1
• ILK2
• ILK-2
• integrin-linked kinase
• integrin-linked kinase-2
• integrin-linked protein kinase
• p59
• p59ILK

Background

Integrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix (ECM). Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ILK