Human IL6R ELISA Kit (Colorimetric)

Sandwich ELISA: Human IL6R ELISA Kit (Colorimetric) [NBP1-89870] - Representative standard curve for human IL-6R ELISA. Human IL-6R was diluted in serial 2-fold steps in IL-6RAssay Buffer (1x). Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

Product Discontinued

Human IL6R ELISA Kit (Colorimetric) Summary

• Description: This ELISA kit has been transitioned to a new version, available here: DVR200
• Specificity: The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-6R positive serum. There was no crossreactivity detected.
• Standard Curve Range: 0.08 - 5.0 ng/mL
• Sensitivity: 0.01 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 2.2%
• Intra-Assay: CV% < 1.7%
• Sample Volume: 20 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL6R

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IL6R ELISA Kit (Colorimetric)

• CD126 antigen
• CD126
• gp80
• IL-6 receptor subunit alpha
• IL-6R 1
• IL-6R subunit alpha
• IL-6R-1
• IL6RA
• IL-6RA
• IL-6R-alpha
• interleukin 6 receptor alpha subunit
• interleukin 6 receptor
• interleukin-6 receptor subunit alpha
• Membrane glycoprotein 80
• MGC104991

Background

Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IL6R ELISA Kit (NBP1-89870)(1)

Publication using NBP1-89870

• Deleon-Pennell KY, Ero OK, Ma Y et al. Glycoproteomic Profiling Provides Candidate Myocardial Infarction Predictors of Later Progression to Heart Failure ACS Omega 2019-01-31 [PMID: 30729226] (ELISA, Human)

Review for IL6R ELISA Kit (NBP1-89870) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 05/25/2017

Summary

• Sample Tested: Human post-MI plasma samples

FAQs for IL6R ELISA Kit (NBP1-89870). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL6R