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Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid, urine

Order Details

IL6R ELISA Kit Summary

Standard Curve Range
0.08 - 5.0 ng/mL
0.01 ng/mL
Kit Type

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.
Concentration is not relevant for this product. Please see the protocols for proper use of this product.


Application Notes
The human sIL-6R ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sIL-6R

Kit Components

  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sIL-6R
  2. HRP-Conjugate anti-human sIL-6R monoclonal antibody
  3. Human sIL-6R Standard lyophilized, 5 ng/ml upon reconstitution
  4. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  5. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  6. Substrate Solution (tetramethyl-benzidine)
  7. Stop Solution (1M Phosphoric acid)
  8. Adhesive Films

Reactivity Notes



Colored dyes for optional use with this kit are available and will be provided free of charge upon request. Please refer to catalog number NBP2-41404.

Alternate Names for IL6R ELISA Kit

  • CD126 antigen
  • CD126
  • gp80
  • IL-6 receptor subunit alpha
  • IL-6R 1
  • IL-6R subunit alpha
  • IL-6R-1
  • IL6RA
  • IL-6RA
  • IL-6R-alpha
  • interleukin 6 receptor alpha subunit
  • interleukin 6 receptor
  • interleukin-6 receptor subunit alpha
  • Membrane glycoprotein 80
  • MGC104991


IL-6 is a multifunctional cytokine involved in the regulation of the immune response, hematopoiesis and acute phase response. It has been recognized to be a member of the alpha-helical cytokine family. IL-6 exerts its action via a cell surface receptor which consists of two subunits, an 80 kDa ligand binding subunit (gp80) of 468 amino acids and a 130 kDa signal transducing protein (gp130) of 896 amino acid residues. Characterisation of the extracellular portion of the 80 kDa IL-6 receptor revealed the existence of a single immunoglobulin-like domain in the NH2-terminal of the extracellular region, which does not contribute to ligand binding. The remainder of the extracellular domain however is essential for low affinity ligand binding, which consecutively triggers the association of the receptor and gp130 thus forming a high affinity binding site for IL-6. A soluble form of the human gp80 protein has been detected in serum and urine samples. This 55 kDa protein representing the extracellular portion of gp80 is generated by shedding, a process that seems to be controlled by protein kinase C. It is still functional, indicating that soluble gp80 plays a biological role in promoting IL-6 activity. So far, the soluble IL-6 receptor is unique in acting as an agonist together with its ligand.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs


Gene Symbol IL6R

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