IL6R Antibody

Product Discontinued

IL6R Antibody Summary

• Immunogen: Recombinant fragment corresponding to a region within amino acids 192 and 458 of IL6 Receptor (Uniprot ID#P08887)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL6R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
• Application Notes: suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Pig (80%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0), 1.0% BSA and 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for IL6R Antibody

• CD126 antigen
• CD126
• gp80
• IL-6 receptor subunit alpha
• IL-6R 1
• IL-6R subunit alpha
• IL-6R-1
• IL6RA
• IL-6RA
• IL-6R-alpha
• interleukin 6 receptor alpha subunit
• interleukin 6 receptor
• interleukin-6 receptor subunit alpha
• Membrane glycoprotein 80
• MGC104991

Background

Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for IL6R Antibody (NBP2-14993). (Showing 1 - 1 of 1 FAQ).

1. In the immunohistochemistry paraffin section protocol...what is the volume of primary antibody that should be added to the section? is it specific for each antibody or a general amount? My antibody is anti il-6 receptor NBP2 14993
   • Thanks for contacting Novus Biologicals technical support. To answer your question, we do not have any specific protocol for NBP2-14993, however the dilution we recommend for IHC-P is 1:100-1:1000. Please note this is a range we recommend and you have to optimize it on your end. You can try three different dilutions for instance, 1:100, 1:500 and 1:1000 to see which one gives the best results. For some of our antibodies we do recommend specific protocols to follow but for NBP2-14993, you can follow our general guideline. I hope this information helps. Please feel free to contact us if you have any further questions.

Bioinformatics

• Gene Symbol: IL6R
• Uniprot:
  • P08887()