IL-9R Antibody

• Reactivity: Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-9R Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat IL‑9 R
  Val38-Ala270
  Accession # EDM04022
• Specificity: Detects rat IL-9 R in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant mouse IL-9 R is observed and approximately 10% cross-reactivity with recombinant human IL-9 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL9R
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-9R Antibody

• CD129 antigen
• CD129
• IL-9 R
• IL-9 receptor
• IL9R
• IL-9R
• interleukin 9 receptor
• interleukin-9 receptor

Background

The IL-9 receptor alpha protein is a member of the type I cytokine receptor family. It is a 62 kDa IL-9 receptor subunit (previously Gfi-2, designated CD129) that binds IL-9 and pairs with the 64 kDa cytokine receptor common gamma -chain to allow cell signaling (1‑3). The 467 amino acid (aa) rat IL-9 R alpha precursor is predicted to contain a 37 aa signal sequence, a 232 aa extracellular domain (ECD) with four conserved cysteine residues in its N-terminal region, a fibronectin type III domain and a WSXWS motif, a 21 aa transmembrane (TM) domain, and a 177 aa cytoplasmic domain with a Box 1 JAK-binding motif (1, 2, 4). A potential isoform contains a 36 aa sequence that is substituted for the TM and cytoplasmic domains (5). The ECD of rat IL-9 R alpha shares 86%, 63%, 66%, 63% and 59% aa identity with the ECD of mouse, human, equine, canine and bovine IL-9 R alpha , respectively. Mast cells, germinal center and B-1b B cells, T cell blasts, and myeloid progenitors express IL-9 R (1, 6‑8). The primary functions of IL-9 have been difficult to determine due to the cross-functionality and cross-induction of cytokines. IL-9 production by regulatory T cells (T-regs), however, is crucial for mast cell recruitment and differentiation, which in turn is crucial for tolerance of allografts (7). Genetic deletion and transgenic expression of IL-9 and other cytokines has also demonstrated the role of IL-9 in mastocytosis, eosinophilia and B cell infiltration of the lung in a mouse asthma-like model, while IL-9 induction of IL-13 is responsible for eosinophil recruitment and mucous upregulation (8‑10).

1. Knoops, L. and J-C. Renauld (2004) Growth Factors 22:207.
2. Flubacher, M.M. et al. (1994) J. Virol. 68:7709.
3. Demoulin, J-B. et al. (1996) Mol. Cell. Biol. 16:4710.
4. Swissprot Accession # Q63216
5. Entrez Accession # EDM04023
6. Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
7. Lu, L-F. et al. (2006) Nature 442:997.
8. Steenwinckel, V. et al. (2007) J. Immunol. 178:3244.
9. Townsend, M.J. et al. (2000) Immunity 13:573.
10. Fallon, P.G. et al. (2002) Immunity 17:7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL9R
• Uniprot:
  • EDM04022()