IL-7R alpha/CD127 Antibody (A7R34) [Phycoerythrin]

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse IL-7 Ra/CD127 PE-conjugated Monoclonal Antibody (Catalog # FAB47742P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

IL-7R alpha/CD127 Antibody (A7R34) [Phycoerythrin] Summary

• Immunogen: Recombinant mouse IL‑7 R alpha /CD127
• Specificity: Detects mouse IL‑7 R alpha /CD127 in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL7R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-7R alpha/CD127 Antibody (A7R34) [Phycoerythrin]

• CD127 antigen
• CD127
• CD127ILRA
• CDW127
• IL-7 R alpha
• IL-7 receptor subunit alpha
• IL7R alpha
• IL-7R alpha
• IL-7R subunit alpha
• IL7R
• IL7RA
• IL-7Ra
• IL-7R-alpha
• interleukin 7 receptor alpha chain
• interleukin 7 receptor isoform H5-6
• interleukin 7 receptor
• interleukin-7 receptor subunit alpha

Background

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature mouse IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type III domain and a WSxWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Within the ECD, mouse IL-7 R alpha shares 67% and 79% aa sequence identity with human and rat IL-7 R alpha , respectively. IL-7 R alpha associates with the common gamma chain ( gamma _c) to form the functional high affinity IL-7 receptor complex (4). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL-7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4^-CD8^-) and CD4⁺ or CD8⁺ single positive T cells as well as on CD8⁺ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mice, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In humans it is required for T cell but not for B cell development (9). IL-7 induces the down regulation and shedding of cell surface IL-7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

 

1. Mazzucchelli, R. and S.K. Durum, 2007, Nat. Rev. Immunol. 7:144.
2. Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193.
3. Goodwin, R.G. et al. (1990) Cell 60:941.
4. Noguchi, M. et al. (1993) Science 262:1877.
5. Barata, J.T. et al. (2006), Exp. Hematol. 34:1133.
6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
13. Reche, P.A. et al. (2001) J. Immunol. 167:336.
14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
15. Jahn, T. et al. (2007) Blood 110:1840.
16. Lai, L. et al. (2006) Blood 107:1776.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL7R