IL-3R beta Antibody [Unconjugated]

Recombinant Mouse IL‑3 (Catalog # 403-ML) stimulates proliferation in the NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-MouseIL‑3 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF549). The ND50 is typically5‑15 µg/mL.

• Reactivity: Mu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-3R beta Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-3 R beta
  His23-Trp440
  Accession # P26954
• Specificity: Detects mouse IL‑3 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Common beta chain is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSF2RB
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-3R beta Antibody [Unconjugated]

• AIC2A
• BetaIl3
• Bil3
• Csfgmrb
• IL3R beta
• IL-3R beta
• IL3R
• IL3RB
• IL-3Rb
• Il3rb2

Background

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta . AIC2A was found to be the result of a gene duplication event. Both the alpha  and the beta  subunits are members of the cytokine receptor superfamily (3).

1. Itoh, N. et al. (1990) Science 247:324.
2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
3. Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-3R beta Antibody (AF549)(3)

Publications using AF549

• M Viaud, O Abdel-Waha, J Gall, S Ivanov, R Guinamard, S Sore, J Merlin, M Ayrault, E Guilbaud, A Jacquel, P Auberger, N Wang, RL Levine, AR Tall, L Yvan-Charv ABCA1 Exerts Tumor-Suppressor Function in Myeloproliferative Neoplasms Cell Rep, 2020-03-10;30(10):3397-3410.e5. 2020-03-10 [PMID: 32160545] (Neutralization, Mouse)
• S Kimura, Y Nakamura, N Kobayashi, K Shiroguchi, E Kawakami, M Mutoh, H Takahashi-, T Yamada, M Hisamoto, M Nakamura, N Udagawa, S Sato, T Kaisho, T Iwanaga, K Hase Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity Nat Commun, 2020-01-13;11(1):234. 2020-01-13 [PMID: 31932605] (ICC/IF, Mouse)
• AF Altenburg, CE van de San, BWS Li, RJ MacLoughli, RAM Fouchier, G van Ameron, A Volz, RW Hendriks, RL de Swart, G Sutter, GF Rimmelzwaa, RD de Vries Modified Vaccinia Virus Ankara Preferentially Targets Antigen Presenting Cells In Vitro, Ex Vivo and In Vivo Sci Rep, 2017-08-17;7(1):8580. 8/17/2017 [PMID: 28819261]

Review for IL-3R beta Antibody (AF549) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Simple Western Mouse 06/04/2019

Summary

• Application: Simple Western
• Sample Tested: Mouse Spleen
• Species: Mouse
• Lot: EHG0217011

Bioinformatics

• Gene Symbol: CSF2RB
• Uniprot:
  • P26954()