IL-36 beta/IL-1F8 Antibody (162601)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
162601
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

IL-36 beta/IL-1F8 Antibody (162601) Summary

Immunogen
E. coli-derived recombinant human IL‑36 beta /IL‑1F8
Met1-Glu157
Accession # NP_775270
Specificity
Detects human IL‑36 beta /IL‑1F8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1ra, rhIL-18, rhIL-36Ra, rhIL-36 alpha, rhIL-1F7 or recombinant mouse IL-36 beta is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL36B
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1099 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-36 beta/IL-1F8 Antibody (162601)

  • family of interleukin 1-eta
  • FIL1 eta
  • FIL1
  • FIL1-(ETA)
  • FIL1H
  • FILI-(ETA)
  • IL-1 eta
  • IL1-ETA
  • IL1F8 (Canonical product IL-1F8a)
  • IL-1F8 (FIL1-eta)
  • IL1F8
  • IL-1F8
  • IL1H2
  • IL-1H2
  • IL36 beta
  • IL-36 beta
  • IL36B
  • interleukin 1 family, member 8 (eta)
  • interleukin 1, eta
  • Interleukin 36, Beta
  • Interleukin-1 eta
  • interleukin-1 family member 8
  • Interleukin-1 homolog 2
  • Interleukin-1 Superfamily e
  • Interleukin-36 Beta

Background

Human interleukin 1 family member #8 [IL-1F8; also named IL-36 beta FIL-1 eta (eta) and IL-1H2] is a member of the IL-1 family of proteins (1‑3, 6). IL-1 family members include IL‑1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -stranded beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Two alternatively spliced transcript variants encode distinct (164 or 157 residues) protein isoforms that differ in their C-terminal 70 amino acid (aa) residues have been reported (3). IL-36 beta isoform 2 is synthesized as a 157 aa protein that contains no signal sequence and no prosegment (1‑2). Unlike IL-36 beta isoform 1 which lacks potential N-linked glycosylation sites, isoform 2 contains one potential N-linked glycosylation site in its unique C-terminus. IL-36 beta is reported to be actively secreted (1). Human IL-36 beta isoform 2 shares 61% aa identity with mouse IL-1 ra, a 183 aa form of IL-36 beta. Within the IL-1 family, IL-36 beta shares 30%, 32%, 37%, 46%, 34%, 45% and 28% aa sequence identity with IL-1 ra, IL-1 beta, IL-36Ra, IL-36 alpha, F7, IL-36 gamma and F10, respectively. Cells reported to express IL-36 beta include resting and activated monocytes and B cells (1, 4). The receptor for IL-36 beta is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-36 beta, along with IL-36 alpha and IL-36 gamma, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

 

  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL36B
Uniprot