IL-36 beta/IL-1F8 Antibody (162601)

• Reactivity: Hu
• Applications: WB
• Clone: 162601
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-36 beta/IL-1F8 Antibody (162601) Summary

• Immunogen: E. coli-derived recombinant human IL‑36 beta /IL‑1F8
  Met1-Glu157
  Accession # NP_775270
• Specificity: Detects human IL‑36 beta /IL‑1F8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-1 alpha , rhIL-1 beta , rhIL-1ra, rhIL-18, rhIL-36Ra, rhIL-36 alpha , rhIL-1F7 or recombinant mouse IL-36 beta is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL36B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-36 beta/IL-1F8 Antibody (162601)

• family of interleukin 1-eta
• FIL1 eta
• FIL1
• FIL1-(ETA)
• FIL1H
• FILI-(ETA)
• IL-1 eta
• IL1-ETA
• IL1F8 (Canonical product IL-1F8a)
• IL-1F8 (FIL1-eta)
• IL1F8
• IL-1F8
• IL1H2
• IL-1H2
• IL36 beta
• IL-36 beta
• IL36B
• interleukin 1 family, member 8 (eta)
• interleukin 1, eta
• Interleukin 36, Beta
• Interleukin-1 eta
• interleukin-1 family member 8
• Interleukin-1 homolog 2
• Interleukin-1 Superfamily e
• Interleukin-36 Beta

Background

Human interleukin 1 family member #8 [IL-1F8; also named IL-36 beta FIL-1 eta (eta) and IL-1H2] is a member of the IL-1 family of proteins (1‑3, 6). IL-1 family members include IL‑1 beta , IL-1 alpha , IL-1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -stranded beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Two alternatively spliced transcript variants encode distinct (164 or 157 residues) protein isoforms that differ in their C-terminal 70 amino acid (aa) residues have been reported (3). IL-36 beta isoform 2 is synthesized as a 157 aa protein that contains no signal sequence and no prosegment (1‑2). Unlike IL-36 beta isoform 1 which lacks potential N-linked glycosylation sites, isoform 2 contains one potential N-linked glycosylation site in its unique C-terminus. IL-36 beta is reported to be actively secreted (1). Human IL-36 beta isoform 2 shares 61% aa identity with mouse IL-1 ra, a 183 aa form of IL-36 beta . Within the IL-1 family, IL-36 beta shares 30%, 32%, 37%, 46%, 34%, 45% and 28% aa sequence identity with IL-1 ra, IL-1 beta , IL-36Ra, IL-36 alpha , F7, IL-36 gamma and F10, respectively. Cells reported to express IL-36 beta include resting and activated monocytes and B cells (1, 4). The receptor for IL-36 beta is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-36 beta , along with IL-36 alpha and IL-36 gamma , has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

 

1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
3. Nicklin, M.J.H. et al. (2002) Genomics 79:718.
4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
5. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-36 beta/IL-1F8 Antibody (MAB1099)(3)

Publications using MAB1099

• T Song, D Spillmann Transcriptomic analysis reveals cell apoptotic signature modified by heparanase in melanoma cells J. Cell. Mol. Med., 2019-05-02;0(0):. 2019-05-02 [PMID: 31044520] (Western Blot, Human)
• Southcombe J, Redman C, Sargent I, Granne I Interleukin-1 family cytokines and their regulatory proteins in normal pregnancy and pre-eclampsia. Clin Exp Immunol, 2015-06-29;181(3):480-90. 2015-06-29 [PMID: 25693732] (Western Blot, Human)
• Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression. J. Immunol., 2011-01-17;186(4):2613-22. 2011-01-17 [PMID: 21242515] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: IL36B
• Uniprot:
  • NP_775270()