Human IL-33 ELISA Kit (Colorimetric)

ELISA: Human IL-33 ELISA Kit (Colorimetric) [NBP2-31048] - Standard curve using the IL-33 standard.

Product Discontinued

Human IL-33 ELISA Kit (Colorimetric) Summary

• Specificity: Measures free IL-33 in the supernatant of activated cells.
• Standard Curve Range: 15-8000 pg/ml
• Sensitivity: 15 pg/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 0.1 ml
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL33

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: For the Detection of Human IL-33 in the Supernatant. The human IL-33 ActivELISA(TM) Kit measures free IL-33 in the supernatant of activated cells. Standard protocols for detecting IL-33 activity include ELISA and Western Blot. The IL-33 ActivELISA(TM) can be completed in one day using a sandwich ELISA protocol. The anti-IL-33 antibody is coated on a plate which then captures free IL-33. The amount of bound IL-33 is detected by adding a second biotinylated anti-IL-33 antibody followed by HRP-conjugated streptavidin. ABTS substrate is then added and the concentration is determined by colorimetric detection in an ELISA plate reader.

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Notes

Additional items required for the ELISA (not included in the IL-33 ActivELISA Module): 96-well ELISA plate reader

Alternate Names for Human IL-33 ELISA Kit (Colorimetric)

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33 is a member of the Interleukin-1 family. It performs dual roles, both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties, as well. It is synthesized as a 30-kDa precursor, which is cleaved by Caspase 1 to generate an 18-kDa fragment, which is sufficient to activate signaling by the IL-33 receptor T1/ST2. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. It directly stimulates eosinophil differentiation from CD117+ progenitors in an IL-5-dependent manner. IL-33/ST2 signaling pathway activates airway eosinophils that exacerbate airway inflammation in an autocrine and paracrine manner. IL-33 signaling through ST2 receptor involves the recruitment of the adaptor protein MyD88, IRAK1, IRAK4, and TRAF6, activating MAPKs and NF-kappaB, thereby regulating the transcription of cytokines typically associated with a Th2 response such as IL-4, IL-5, and IL-13 (Foo Y et al, 2011). The affinity of IL-33 for ST2L is enhanced in the presence of IL-1RAcP. IL-33 plays a significant role in the amplification of AAM polarization and chemokine production, which contribute to innate and Ag-induced airway inflammation. It strongly amplifies the expression of Arginase I and Ym1 as well as the production of CCL24 and CCL17, which may further recruit inflammatory cells. It also enhanced the eosinophil-mediated differentiation of airway macrophages toward the alternatively activated macrophage phenotype in an IL-13-dependent manner. IL-33 induces TLR4 expression on macrophages, rendering them more responsive to LPS stimulation. Unprocessed IL-33 accumulates in the nucleus, where it acts as a transcriptional repressor. IL-33 orchestrates its potential role as a mediator in the pathophysiology of many diseases such as cardiovascular disorder, asthma and rheumatoid arthritis (Bartosz Stolarski et al, 2011).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IL-33 ELISA Kit (NBP2-31048)(2)

Publications using NBP2-31048

• Vossenkamper A, Marches O, Fairclough PD et al. Inhibition of NF-kB signaling in human dendritic cells by the enteropathogenic Escherichia coli effector protein NleE. J Immunol. 2010-10-01 [PMID: 20833837] (Human)
  
  Details:
  Nuclear and cytosolic extracts from monocyte derived human dendritic cells in the presence of EPEC LPS, Fig 3D.
• Kondo A, Yamashita T, Tamura H et al. Interferon-gamma and tumor necrosis factor-alpha induce an immunoinhibitory molecule, B7-H1, via nuclear factor-kappaB activation in blasts in myelodysplastic syndromes. Blood. 2010-08-19 [PMID: 20472834]
  
  Details:
  NF-kB/p65 ActivELISA (IMK-503) kit and Nuclear extraction kit (10081K). SKM-1 leukemic cells were separated into cytoplasmic and nuclear fractions then subject to western blot analysis, Fig 2A &B.

FAQs for IL-33 ELISA Kit (NBP2-31048). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL33