IL-3 Antibody (MP28F8) [Biotin]

• Reactivity: Mu
• Applications: WB, B/N, ELISA, ELISA(Cap), ELISA(Det)
• Clone: MP28F8
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin

IL-3 Antibody (MP28F8) [Biotin] Summary

• Immunogen: COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse IL-3
  Accession # P01586
• Specificity: Detects mouse IL-3 in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human (rh) IL‑3 or recombinant rat IL-3 is observed. Does not neutralize the biological activity of rhIL-3.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL3
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Neutralization
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Alternate Names for IL-3 Antibody (MP28F8) [Biotin]

• Hematopoietic growth factor
• IL3
• IL-3
• IL-3MGC79398
• interleukin 3 (colony-stimulating factor, multiple)
• interleukin-3
• Mast cell growth factor
• mast-cell growth factor
• MCGF
• MCGFMGC79399
• MULTI-CSF
• multilineage-colony-stimulating factor
• Multipotential colony-stimulating factor
• P-cell stimulating factor
• P-cell-stimulating factor

Background

IL-3 (Interleukin-3, Hematopoietic growth factor, Mast cell growth factor), a pleiotropic growth factor, is mainly produced by activated T-cells, but is also produced by keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. IL-3 promotes proliferation and differentiation of various hematopietic cell types, including pluripotent stem cells and more committed progenitors. IL-3 also affects activity of mature hematopoietic cells and often functions in combination with other cytokines. Human IL-3 is species-specific and shows no biological activity on murine cells. Recombinant human IL-3 is a 17 kDa globular protein consisting of 133 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL3