IL-3 Antibody (MP28F8) [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap), ELISA(Det)
• Clone: MP28F8
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

IL-3 Antibody (MP28F8) [Alexa Fluor® 647] Summary

• Immunogen: COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse IL-3
  Accession # P01586
• Specificity: Detects mouse IL-3 in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human (rh) IL‑3 or recombinant rat IL-3 is observed. Does not neutralize the biological activity of rhIL-3.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-3 Antibody (MP28F8) [Alexa Fluor® 647]

• Hematopoietic growth factor
• IL3
• IL-3
• IL-3MGC79398
• interleukin 3 (colony-stimulating factor, multiple)
• interleukin-3
• Mast cell growth factor
• mast-cell growth factor
• MCGF
• MCGFMGC79399
• MULTI-CSF
• multilineage-colony-stimulating factor
• Multipotential colony-stimulating factor
• P-cell stimulating factor
• P-cell-stimulating factor

Background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta  subunits. The IL-3 R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta  subunits, AIC2A ( beta _IL-3) and AIC2B ( beta _c) are present in mouse cells. beta _IL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha  subunit. The beta _c subunits does not bind any cytokine but forms functional high affinity receptors with the alpha  subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.