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403-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-403-NA). The ND50 is typically 0.01-0.03 µg/mL." class="big_lightbox" target="_blank">
403-ML) was serially diluted and captured by Rat Anti-Mouse IL‑3 Monoclonal Antibody (Catalog # MAB403) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-403-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox" target="_blank">
203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-3 Antigen Affinity-purified Polyclonal Anti-body (Catalog # AF-203-NA). The ND50 is typically 0.05-0.25 µg/mL." class="big_lightbox" target="_blank">
203-IL) was serially diluted and captured by Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-203-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
203-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB203). The ND50 is typically 0.03-0.08 µg/mL." class="big_lightbox" target="_blank">
BT-003-GMP, BT-003-AFL, 203-IL; 50 ng/mL), SCF (Catalog #s BT-SCF-GMP, BT-SCF-AFL, BT-SCF; 100 ng/mL), TPO (Catalog #s BT-TPO-GMP, BT-TPO-AFL, BT-TPO; 100 ng/mL), FLT-3L (Catalog # BT-FT3L-GMP, BT-FT3L-AFL, BT-FT3L; 100 ng/mL), and SR1 (Catalog # 7086, 1 µM). After 7 days, frequency (%) of CD34+ cells was analyzed by flow cytometry with an anti-human CD34 monoclonal antibody (Catalog # MAB7227). CD34+ Cell Expansion is calculated by multiplying Total Nucleated Cell (TNC) fold expansion by the percent CD34+ cells. Cell viability on Day 7 was greater than 90% for all conditions." class="big_lightbox" target="_blank">
BT-003-GMP), Animal-Free (Catalog # BT-003-AFL) and RUO (203-IL) grades of Recombinant Human IL-3 as measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. (orange, green, red, respectively)." class="big_lightbox" target="_blank">
BT-003-GMP, BT-003-AFL, 203-IL; 50 ng/mL), SCF (Catalog #s BT-SCF-GMP, BT-SCF-AFL, BT-SCF; 100 ng/mL), TPO (Catalog #s BT-TPO-GMP, BT-TPO-AFL, BT-TPO; 100 ng/mL), FLT-3L (Catalog # BT-FT3L-GMP, BT-FT3L-AFL, BT-FT3L; 100 ng/mL), and SR1 (Catalog # 7086, 1 µM). After 7 days, frequency (%) of CD34+ cells was analyzed by flow cytometry with an anti-human CD34 monoclonal antibody (Catalog # MAB7227). CD34+ Cell Expansion is calculated by multiplying Total Nucleated Cell (TNC) fold expansion by the percent CD34+ cells. Cell viability on Day 7 was greater than 90% for all conditions." class="big_lightbox" target="_blank">
IL-3 (Interleukin-3, Hematopoietic growth factor, Mast cell growth factor), a pleiotropic growth factor, is mainly produced by activated T-cells, but is also produced by keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. IL-3 promotes proliferation and differentiation of various hematopietic cell types, including pluripotent stem cells and more committed progenitors. IL-3 also affects activity of mature hematopoietic cells and often functions in combination with other cytokines. Human IL-3 is species-specific and shows no biological activity on murine cells. Recombinant human IL-3 is a 17 kDa globular protein consisting of 133 amino acid residues.
![Immunocytochemistry IL-3 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af-203-na_human-il-3-affinity-purified-polyclonal-ab-immunocytochemistry-251202213158.jpg)
![Neutralization IL-3 Antibody (4806) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-3_MAB203_Block_Neutralize_9056.jpg)
![Sandwich ELISA IL-3 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/IL-3-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79374-img0001.jpg)
![N/A IL-3 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL3_D3000_ELISA_546.jpg)
![N/A IL-3 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL3_M3000_ELISA_931.jpg)
![N/A IL-3 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL3_DY403_ELISA_2000.jpg)
![SEC-MALS IL-3 [Unconjugated]](http://images.novusbio.com/fullsize/203-il_recombinant-human-il-3-protein-sec-mals-133202482656..jpg)
![SEC-MALS IL-3 [Unconjugated]](http://images.novusbio.com/fullsize/403-ml_recombinant-mouse-il-3-protein-sec-mals-213202484921..jpg)
![Bioactivity IL-3 [Unconjugated]](http://images.novusbio.com/images/bt-003-afl_recombinant-human-il-3-protein-animal-free-bioactivity-1572025161945..jpg)
![Neutralization IL-3 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL-3_AF-203-NA_Block_Neutralize_8641.jpg)
![ELISA IL-3 Antibody [Unconjugated]](http://images.novusbio.com/images/af-203-na_human-il-3-affinity-purified-polyclonal-ab-elisa-639201852921071786.jpg)
![Immunocytochemistry IL-3 Antibody (4806) [Unconjugated]](http://images.novusbio.com/images/antibody/IL3_MAB203_Immunocytochemistry__Immunofluorescence_17404.jpg)
![N/A IL-3 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL3_D3000_ELISA_547.jpg)
![N/A IL-3 [HRP]](http://images.novusbio.com/fullsize/elisa/IL-3_D3000_ELISA_19.jpg)
![N/A IL-3 [HRP]](http://images.novusbio.com/fullsize/elisa/IL-3_M3000_ELISA_234.jpg)
![Bioactivity IL-3 [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL-3_203-IL_347.jpg)
![SDS-Page IL-3 [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL-3_203-IL_345.jpg)
![Data IL-3 [Unconjugated]](http://images.novusbio.com/images/203-il_recombinant-human-il-3-protein-data-253202610550.jpg)
![Bioactivity IL-3 [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL-3_403-ML_396.jpg)
![SDS-Page IL-3 [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL-3_403-ML_394.jpg)
![Bioactivity IL-3 [Unconjugated]](http://images.novusbio.com/fullsize/bt-003-afl_recombinant-human-il-3-protein-animal-free-bioactivity-157202516249.jpg)
![SDS-Page IL-3 [Unconjugated]](http://images.novusbio.com/fullsize/bt-003-afl_recombinant-human-il-3-protein-animal-free-sds-page-157202516439.jpg)
![Data IL-3 [Unconjugated]](http://images.novusbio.com/images/bt-003-afl_recombinant-human-il-3-protein-animal-free-data-253202610428.jpg)
