IL-27R alpha/WSX-1/TCCR Antibody (191106) [Phycoerythrin]

Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A) and either (A) Mouse Anti-Human IL-27 Ra/WSX-1/TCCR PE-conjugated Monoclonal Antibody (Catalog # FAB14791P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (IC0041P). View our protocol for Staining Membrane-associated Proteins.

U937 cells were stained with Mouse Anti-Human IL‑27 R alpha /WSX‑1/TCCR PE‑conjugated Monoclonal Antibody (Catalog # FAB14791P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 191106
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-27R alpha/WSX-1/TCCR Antibody (191106) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-27 R alpha /WSX‑1/TCCR
  Gly34-Lys516
  Accession # Q6UWB1
• Specificity: Detects human IL-27 R alpha /WSX‑1/TCCR in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human gp130 or recombinant mouse IL-27 Ra is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL27RA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-27R alpha/WSX-1/TCCR Antibody (191106) [Phycoerythrin]

• class I cytokine receptor
• CRL1IL-27R
• Cytokine receptor-like 1
• IL-27 R alpha
• IL27R alpha
• IL27R
• IL27RA
• IL-27Ra
• IL-27R-alpha
• interleukin 27 receptor, alpha
• interleukin-27 receptor subunit alpha
• TCCR
• TCCRIL-27 receptor subunit alpha
• T-cell cytokine receptor type 1
• Type I T-cell cytokine receptor
• WSX-1
• WSX1IL-27R subunit alpha
• zcytor1

Background

IL‑27 R alpha (also known as WSX‑1 and TCCR) is a 96‑100 kDa member of the type I, group 2 cytokine receptor family (1 - 6). Mature IL‑27 R alpha  is a type I transmembrane glycoprotein that contains a 484 amino acid (aa) extracellular region, a 21 aa transmembrane segment and a 99 aa cytoplasmic domain. Consistent with type I cytokine receptors, the extracellular region contains four positionally conserved cysteine residues, a WSxWS motif (for receptor folding and ligand binding), and three fibronectin type III repeats. The intracellular domain contains a "box‑1" motif that may be involved with Janus kinases (3). One potential alternate splice form has been hypothesized that involves a 58 aa addition to the cytoplasmic domain and, based on mouse, a soluble 33 kDa splice form that shows a 20 aa substitution for aa 257‑636 may also occur in human (3, 7). The human IL‑27 R alpha extracellular region shares 63% amino acid identity with the mouse IL‑27 R alpha extracellular domain (2, 3). IL‑27 R alpha is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and naïve T cells (1, 2, 4, 8). Typical of other class I cytokine receptor chains, the ligand binding IL‑27 R alpha molecule is known to heterodimerize with a signal‑transducing subunit (gp130) to form a functional IL‑27 receptor (9, 10). In addition, IL‑27 R alpha is reported to complex with CNTFR alpha and gp130 form a humanin receptor on neurons (7, 11), and to complex with gp130 and IL‑6 R to form a receptor for a p28:CLF heterodimeric cytokine on lymphocytes (12). Studies using IL‑27 R alpha /WSX‑1^‑/‑ mice reveal that IL‑27 has the ability to suppress T cell activity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity (4, 13, 14). In particular, IL‑27 is known to act on naïve T cells, blocking their differentiation into a Th17 phenotype. Notably, cells committed to a Th17 phenotype, although they express a functional IL‑27 receptor, are unresponsive to the effects of IL‑27 (15). Activated T cells that are CD4⁺ and CD8⁺, and which express the IL‑27 receptor, can be induced by IL‑27 to form a double‑positive CD25⁺ FoxP3^‑ IFN‑ gamma plus IL‑10 secreting phenotype that both promotes and suppresses the inflammatory response (16).

1. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
2. Chen, Q. et al. (2000) Nature 407:916.
3. Sprecher, C.A. et al. (1998) Biochem. Biophys. Res. Commun. 246:82.
4. Artis, D. et al. (2004) J. Immunol. 173:5626.
5. Yoshida, H. & Y. Miyazaki (2008) Int. J. Biochem. Cell Biol. 40:2379.
6. Yoshida, H. & M. Yoshiyuki (2008) Immunol. Rev. 226:234.
7. Hashimoto, Y. et al. (2009) Biochem. Biophys. Res. Commun. 389:95.
8. Holscher, C. et al. (2005) J. Immunol. 174:3534.
9. Pflanz, S. et al. (2004) J. Immunol. 172:2225.
10. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
11. Hashimoto, Y. et al. (2009) Mol. Biol. Cell 20:2864.
12. Crabe, S. et al. (2009) J. Immunol. 183:7692.
13. Villarino, A. et al. (2003) J. Immunol. 170:645.
14. Hamano., S. et al. (2003) Immunity 19:657.
15. El-behi, M. et al. (2009) J. Immunol. 183:4957.
16. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-27R alpha/WSX-1/TCCR Antibody (FAB14791P)(17)

Publications using FAB14791P

• Martin, E;Winter, S;Garcin, C;Tanita, K;Hoshino, A;Lenoir, C;Fournier, B;Migaud, M;Boutboul, D;Simonin, M;Fernandes, A;Bastard, P;Le Voyer, T;Roupie, AL;Ben Ahmed, Y;Leruez-Ville, M;Burgard, M;Rao, G;Ma, CS;Masson, C;Soudais, C;Picard, C;Bustamante, J;Tangye, SG;Cheikh, N;Seppänen, M;Puel, A;Daly, M;Casanova, JL;Neven, B;Fischer, A;Latour, S; Role of IL-27 in Epstein-Barr virus infection revealed by IL-27RA deficiency Nature 2024-03-20 [PMID: 38509369] (Flow Cytometry, Human)
• Yu Zhang, Sixi Wei, Qingqing Wu, Xue Shen, Wanrong Dai, Zhiqi Zhang et al. Interleukin-35 promotes Breg expansion and interleukin-10 production in CD19+ B cells in patients with ankylosing spondylitis Clinical Rheumatology 8/1/2022 [PMID: 35420296]
• Jie Cheng, Timothy G. Myers, Callie Levinger, Princy Kumar, Jai Kumar, Bruktawit A. Goshu et al. IL-27 induces IFN/STAT1-dependent genes and enhances function of TIGIT+ HIVGag-specific T cells iScience 1/21/2022 [PMID: 35005538]
• Arshi Khanam, Natarajan Ayithan, Lydia Tang, Bhawna Poonia, Shyam Kottilil IL-21–Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis B Frontiers in Immunology 1/28/2021 [PMID: 33584666]
• MC Cavé, S Maillard, K Hildenbran, C Mamelonet, MJ Feige, O Devergne Glycosaminoglycans bind human IL-27 and regulate its activity Eur. J. Immunol., 2020-07-02;0(0):. 2020-07-02 [PMID: 32483835] (Flow Cytometry, Human)
• O Goovaerts, M Massinga-L, P Ondoa, A Ceulemans, W Worodria, H Mayanja-Ki, R Colebunder, L Kestens Increased KLRG1 and PD-1 expression on CD8 T lymphocytes in TB-IRIS PLoS ONE, 2019-04-25;14(4):e0215991. 2019-04-25 [PMID: 31022273] (Flow Cytometry, Human)
• C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies J. Leukoc. Biol., 2018-07-24;0(0):. 2018-07-24 [PMID: 30040142] (Flow Cytometry, Human)
• F Xu, J Yi, Z Wang, Y Hu, C Han, Q Xue, X Zhang, X Luan IL-27 regulates the adherence, proliferation, and migration of MSCs and enhances their regulatory effects on Th1 and Th2 subset generations Immunol. Res., 2017-08-01;0(0):. 2017-08-01 [PMID: 28612255] (Flow Cytometry, Human)
• IL-27 Directly Enhances Germinal Center B Cell Activity and Potentiates Lupus in Sanroque Mice J Immunol, 2016-09-12;0(0):. 2016-09-12 [PMID: 27619997] (Flow Cytometry, Human)
• Dietrich C, Candon S, Ruemmele F, Devergne O A soluble form of IL-27Ralpha is a natural IL-27 antagonist. J Immunol, 2014-04-25;192(11):5382-9. 2014-04-25 [PMID: 24771852] (Flow Cytometry, Human)
• Morandi F, Airoldi I, Pistoia V IL-27 driven upregulation of surface HLA-E expression on monocytes inhibits IFN-gamma release by autologous NK cells. J Immunol Res, 2014-03-10;2014(0):938561. 2014-03-10 [PMID: 24741633] (Flow Cytometry, Human)
• Morandi , Fabio, Di Carlo , Emma, Ferrone , Soldano, Petretto , Andrea, Pistoia , Vito, Airoldi , Irma IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol, 2014-02-19;192(6):2634-42. 2014-02-19 [PMID: 24554774] (Flow Cytometry, Human)
• Tzeng , Tsai-Ten, Tsay , Huey-Jen, Chang , Luping, Hsu , Chia-Lin, Lai , Tzu-Hsua, Huang , Fong-Lee, Shiao , Young-Ji Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid. J Biomed Sci, 2013-12-06;20(0):90. 2013-12-06 [PMID: 24313976] (Flow Cytometry, Human)
• Kachroo P, Lee M, Zhang L, Baratelli F, Lee G, Srivastava M, Wang G, Walser T, Krysan K, Sharma S, Dubinett S, Lee J IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer. J Exp Clin Cancer Res, 2013-11-25;32(0):97. 2013-11-25 [PMID: 24274066] (Flow Cytometry, Human)
• Chen , Qian, Swaminathan , Sanjay, Yang , De, Dai , Lue, Sui , Hongyan, Yang , Jun, Hornung , Ronald L, Wang , Yanmei, Huang , Da Wei, Hu , Xiaojun, Lempicki , Richard, Imamichi , Tomozumi Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway. PLoS ONE, 2013-03-20;8(3):e59194. 2013-03-20 [PMID: 23527130] (Flow Cytometry, Human)
• Guzzo C, Hopman W, Che Mat N, Wobeser W, Gee K IL-27-Induced Gene Expression Is Downregulated in HIV-Infected Subjects. PLoS ONE, 2012-09-25;7(9):e45706. 2012-09-25 [PMID: 23049843] (Flow Cytometry, Human)
• Cocco C, Di Carlo E, Zupo S Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo. Leukemia, 2011-12-23;26(6):1365-74. 2011-12-23 [PMID: 22193967] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL27RA
• Uniprot:
  • Q6UWB1()