IL-24 Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, B/N, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

IL-24 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑24
  Gln50-Leu206
  Accession # Q2YHE5
• Specificity: Detects human IL-24 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-24 Antibody [Alexa Fluor® 750]

• C49A
• FISP
• IL10B
• IL24
• IL-24
• interleukin 24
• MDA-7
• MDA7MOB5
• melanocyte-associated Mda-7
• melanoma differentiation association protein 7
• Melanoma differentiation-associated gene 7 protein
• MOB-5
• ST16
• ST16interleukin-24
• suppression of tumorigenicity 16 (melanoma differentiation)
• Suppression of tumorigenicity 16 protein

Background

Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids that contains a 48 amino acid (aa) signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35-40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4⁺ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL-24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN-gamma , IL-1 beta , IL‑12, and TNF-alpha . At high concentrations, it is a strong inducer of apoptosis in tumor cells but not normal cells. IL-24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL-20 R alpha and IL-20 R beta , which is shared with IL-19 and IL-20. The second complex is a combination of IL-22 R and IL-20 R beta , which is shared with IL-20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.