IL-1ra/IL-1F3/IL1RN Antibody [Unconjugated]


Western blot shows lysates of RT-4 human bladder carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL‑1ra/IL‑1F3 Antigen more
Simple Western lane view shows lysates of RT-4 human bladder carcinoma cell line, A431 human epithelial carcinoma cell line, human skin cancer tissue, and mouse skin cancer tissue, loaded at 0.2 mg/mL. A more
IL‑1ra/IL‑1F3 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) at 5 µg/mL more
Recombinant HumanIL‑1ra/IL‑1F3 (Catalog #280‑RA) inhibits Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog #200‑LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, B/N

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IL-1ra/IL-1F3/IL1RN Antibody [Unconjugated] Summary

E. coli-derived recombinant human IL‑1ra/IL-1F3
Accession # P18510
Detects human IL‑1ra/IL-1F3 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 15% cross-reactivity with recombinant mouse IL-1ra is observed.
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.5 ug/mL
  • Simple Western 20 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization ≤4 ug/mL
Read Publications using
AF-280-NA in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1ra/IL-1F3/IL1RN Antibody [Unconjugated]

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4


IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1ra/IL-1F3/IL1RN Antibody (AF-280-NA)(9)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 4 applications: ICC, IHC, Neutralization, Western Blot.

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Showing Publications 1 - 9 of 9.
Publications using AF-280-NA Applications Species
DJ Kota, KS Prabhakara, N Toledano-F, D Bhattarai, Q Chen, B DiCarlo, P Smith, F Triolo, PL Wenzel, CS Cox, SD Olson Prostaglandin E2 Indicates Therapeutic Efficacy Of Mesenchymal Stem Cells In Experimental Traumatic Brain Injury Stem Cells, 2017;0(0):. 2017 [PMID: 28233425] (Western Blot, Human) Western Blot Human
JH Lee, EH Kam, JM Kim, SY Kim, EJ Kim, SY Cheon, BN Koo Intranasal Administration of Interleukin-1 Receptor Antagonist in a Transient Focal Cerebral Ischemia Rat Model Biomol Ther (Seoul), 2017;25(2):149-157. 2017 [PMID: 27530114] (IHC, Rat) IHC Rat
Rider P, Carmi Y, Yossef R, Guttman O, Eini H, Azam T, Dinarello C, Lewis E IL-1 Receptor Antagonist Chimeric Protein: Context-Specific and Inflammation-Restricted Activation. J Immunol, 2015;195(4):1705-12. 2015 [PMID: 26157171] (Western Blot, Human) Western Blot Human
Megjugorac NJ, Gallagher GE, Gallagher G IL-4 enhances IFN-lambda1 (IL-29) production by plasmacytoid DCs via monocyte secretion of IL-1Ra. Blood, 2010;115(21):4185-90. 2010 [PMID: 20233967] (Neutralization, Human) Neutralization Human
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Dig. Dis. Sci., 2007;52(10):2775-83. 2007 [PMID: 17394066] (ICC, Human) ICC Human
Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells. Br. J. Pharmacol., 2007;151(1):115-27. 2007 [PMID: 17351655] (Western Blot, Human) Western Blot Human
Grundtman C, Salomonsson S, Dorph C, Bruton J, Andersson U, Lundberg IE Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies. Arthritis Rheum., 2007;56(2):674-87. 2007 [PMID: 17265504] (ICC, Human) ICC Human
Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R IL-22 increases the innate immunity of tissues. Immunity, 2004;21(2):241-54. 2004 [PMID: 15308104] (Neutralization, Human) Neutralization Human
Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta. J. Allergy Clin. Immunol., 2002;110(4):624-33. 2002 [PMID: 12373272] (Neutralization, Human) Neutralization Human

Reviews for IL-1ra/IL-1F3/IL1RN Antibody (AF-280-NA) (0)

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Secondary Antibodies


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Other Available Formats

Biotin BAF280

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Gene Symbol IL1RN