IL-1ra/IL-1F3/IL1RN Antibodies

HAF017). A specific band was detected for IL-1ra/IL-1F3 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for IL-1ra/IL-1F3 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RT-4 human bladder carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1ra/IL-1F3 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA). The ND₅₀ is typically ≤ 4 µg/mL." class="big_lightbox"> 280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA). The ND₅₀ is typically ≤ 4 µg/mL." class="big_thumb" />

280-RA) was serially diluted and captured by Mouse Anti-Human IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB280) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 280-RA) was serially diluted and captured by Mouse Anti-Human IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB280) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

480-RM) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Mouse IL-1ra/IL-1F3 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox"> 480-RM) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Mouse IL-1ra/IL-1F3 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA). The ND₅₀ is typically 1-5 µg/mL." alt="Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Mouse IL-1ra/IL-1F3 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

480-RM) was serially diluted and captured by Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 480-RM) was serially diluted and captured by Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

480-RM) was serially diluted and captured by Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 480-RM) was serially diluted and captured by Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB280). The ND₅₀ is typically 10-20 µg/mL." class="big_lightbox"> 280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB280). The ND₅₀ is typically 10-20 µg/mL." alt="Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB280). The ND₅₀ is typically 10-20 µg/mL." class="big_thumb" />

280-RA) was serially diluted and captured by Mouse Anti-Human IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB280) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 280-RA) was serially diluted and captured by Mouse Anti-Human IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB280) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

780-RA) inhibits Recombinant Porcine IL-1a/IL-1F1 (Catalog # 680-PI) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Porcine IL-1a/ IL-1F1 (75 pg/mL) activity elicited by Recombinant Porcine IL-1ra/ IL-1F3 (0.2 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Porcine IL-1ra/IL-1F3 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF780). The ND₅₀ is typically 3-15 µg/mL." class="big_lightbox"> 780-RA) inhibits Recombinant Porcine IL-1a/IL-1F1 (Catalog # 680-PI) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Porcine IL-1a/ IL-1F1 (75 pg/mL) activity elicited by Recombinant Porcine IL-1ra/ IL-1F3 (0.2 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Porcine IL-1ra/IL-1F3 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF780). The ND₅₀ is typically 3-15 µg/mL." alt="Recombinant Porcine IL-1ra/ IL-1F3 (Catalog # 780-RA) inhibits Recombinant Porcine IL-1a/IL-1F1 (Catalog # 680-PI) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Porcine IL-1a/ IL-1F1 (75 pg/mL) activity elicited by Recombinant Porcine IL-1ra/ IL-1F3 (0.2 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Porcine IL-1ra/IL-1F3 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF780). The ND₅₀ is typically 3-15 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-1ra/IL-1F3 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Equine IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2466) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

2466-RA) was serially diluted and captured by Goat Anti-Equine IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2466) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2466) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2466-RA) was serially diluted and captured by Goat Anti-Equine IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2466) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2466) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2466-RA) was serially diluted and captured by Goat Anti-Equine IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2466) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2466) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2466-RA) was serially diluted and captured by Goat Anti-Equine IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2466) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2466) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF005). A specific band was detected for IL-1ra/IL-1F3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for IL-1ra/IL-1F3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line treated with 1 µg/mL LPS for 24 hours. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB4801) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-1ra/IL-1F3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Polyclonal Antibody (Catalog # AB-280-NA). The ND₅₀ is typically 5-10 µg/mL." class="big_lightbox"> 280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Polyclonal Antibody (Catalog # AB-280-NA). The ND₅₀ is typically 5-10 µg/mL." alt="Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Polyclonal Antibody (Catalog # AB-280-NA). The ND₅₀ is typically 5-10 µg/mL." class="big_thumb" />