IL-18/IL-1F4 Antibody (74801) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) either untreated (upper panel) or treated with LPS for 24 hours (lower panel) were stained with Mouse Anti-Human IL-18/IL-1F4 PE-conjugated Monoclonal Antibody (Catalog # IC646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

THP-1 human monocytic leukemia cell line was stained with Mouse Anti-Human IL-18/IL-1F4 PE-conjugated Monoclonal Antibody (Catalog # IC646P, filled histogram) or isotype control antibody (IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 74801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-18/IL-1F4 Antibody (74801) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human IL‑18/IL‑1F4
  Accession # Q14116
• Specificity: Detects the pro region of human IL-18/IL-1F4 in direct ELISAs and Western blots. Does not cross‑react with recombinant human IL-18, recombinant mouse IL-18 or recombinant rat IL-18.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL18
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18/IL-1F4 Antibody (74801) [Phycoerythrin]

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

Pro-IL-18 (pro-Interleukin 18; also known as pro-IGIF and pro-IL-1 gamma ) is a 24 kDa member of the IL-1 family of molecules. It is widely expressed, being produced by keratinocytes, intestinal epithelium, T cells, macrophages and osteoblasts. Human Pro-IL-18 is 193 amino acids (aa) in length. Although mature IL-18 induces IFN-gamma secretion by NK and T cells, Pro-IL-18 appears to have little intrinsic activity. Generally, active IL-18 is considered to arise from caspase-1 cleavage of Pro-IL-18 between Asp36-Tyr37. This generates an 18 kDa mature C-terminal fragment, and a 4 kDa (predicted) N-terminal prosegment that runs at 6 kDa in SDS-PAGE. Other proteases are known to process Pro-IL-18. Caspase-3 cleavage after Asp68 generates an inactive 14 kDa mature segment, Merpin beta -subunit cleavage after Asn52 generates a marginally active 17 kDa mature segment, while parasite Cys protease cleavage after Val47 generates an inactive 17 kDa mature molecule. One splice variant shows a deletion of aa 27-30. Over aa 2-36, human Pro-IL-18 shares 63% aa identity with mouse Pro-IL-18.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18/IL-1F4 Antibody (IC646P)(5)

Publications using IC646P

• S Liu, L Yang, S Jia, R Zhao, Z Jin Interleukin-35 suppresses the activity of natural killer-like B cells in patients with hepatocellular carcinoma International immunopharmacology, 2021-09-20;100(0):108161. 2021-09-20 [PMID: 34555643] (Flow Cytometry, Human)
• Z Wang, Z Liu, L Wang, J Wang, L Chen, H Xie, H Zhang, S He Altered expression of IL-18 binding protein and IL-18 receptor in basophils and mast cells of asthma patients Scand. J. Immunol., 2018-04-10;0(0):. 2018-04-10 [PMID: 29505668] (Flow Cytometry, Human)
• LA Callender, EC Carroll, RWJ Beal, ES Chambers, S Nourshargh, AN Akbar, SM Henson Human CD8(+) EMRA T cells display a senescence-associated secretory phenotype regulated by p38 MAPK Aging Cell, 2017-10-12;0(0):. 2017-10-12 [PMID: 29024417] (Flow Cytometry, Human)
• H Zhang, J Wang, L Wang, H Xie, L Chen, S He Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R J. Cell. Mol. Med., 2017-09-18;0(0):. 2017-09-18 [PMID: 28922563] (Flow Cytometry, Human)
• Y Hu, J Wang, H Zhang, H Xie, W Song, Q Jiang, N Zhao, S He Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells Mediators Inflamm., 2017-08-03;2017(0):3090782. 2017-08-03 [PMID: 28839348] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL18
• Uniprot:
  • Q14116()